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Title: 3D Centrifugation‐Enabled Priming of Synaptic Activation Promotes Primary T Cell Expansion
Abstract Autologous cell therapy depends on T lymphocyte expansion efficiency and is hindered by suboptimal interactions between T cell receptors (TCR) and peptide‐MHC molecules. Various artificial antigen presenting cell systems that enhance these interactions are often labor‐intensive, fabrication costly, highly variable, and potentially unscalable toward clinical setting. Here, 3D centrifugation‐enabled priming of T cell immune‐synapse junctions is performed to generate tight T cell–Dynabead aggregates at a rate 200‐fold faster than that of conventional 24‐h bulk shaking. Furthermore, by forming T cell–Dynabead aggregates in the starting culture, two‐ to sixfold greater T cell expansion is achieved over conventional T cell expansion for cancer patient‐derived primary T cells while limiting over‐activation. Creating 3D T cell–Dynabead aggregates as the “booster” material enables highly efficient polyclonal T cell expansion without the need for complex surface modification of artificial antigen‐presenting cells (APCs). This method can be modularly adapted to existing T cell expansion processes for various applications, including adoptive cell therapies (ACTs).  more » « less
Award ID(s):
1841509 1841473
PAR ID:
10490319
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
Advanced Science News
Date Published:
Journal Name:
Advanced Therapeutics
Volume:
6
Issue:
12
ISSN:
2366-3987
Subject(s) / Keyword(s):
adoptive cell therapy (ACT), high-throughput mechanobiology, T cell activation, T cell expansion, T cell receptor (TCR)
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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