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Title: Genome streamlining to improve performance of a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973
Genome streamlining is an evolutionary strategy used by natural living systems to dispense unnecessary genes from their genome as a mechanism to adapt and evolve. While this strategy has been successfully borrowed to develop synthetic heterotrophic microbial systems with desired phenotype, it has not been extensively explored in photoautotrophs. Genome streamlining strategy incorporates both computational predictions to identify the dispensable regions and experimental validation using genome-editing tool, and in this study, we have employed a modified strategy with the goal to minimize the genome size to an extent that allows optimal cellular fitness under specified conditions. Our strategy has explored a novel genome-editing tool in photoautotrophs, which, unlike other existing tools, enables large, spontaneous optimal deletions from the genome. Our findings demonstrate the effectiveness of this modified strategy in obtaining strains with streamlined genome, exhibiting improved fitness and productivity.  more » « less
Award ID(s):
2037887 2037829
PAR ID:
10492392
Author(s) / Creator(s):
; ; ; ; ; ; ; ;
Editor(s):
Harwood, Caroline S.
Publisher / Repository:
ASM Journals
Date Published:
Journal Name:
mBio
ISSN:
2150-7511
Subject(s) / Keyword(s):
CRISPR-Cas3 genome minimization cyanobacteria large progressive deletion essential gene identification
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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