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  • P ool P arty 2: An integrated pipeline for analysing pooled or indexed low‐coverage whole‐genome sequencing data to discover the genetic basis of diversity
Title: P ool P arty 2: An integrated pipeline for analysing pooled or indexed low‐coverage whole‐genome sequencing data to discover the genetic basis of diversity
Abstract Whole‐genome sequencing data allow survey of variation from across the genome, reducing the constraint of balancing genome sub‐sampling with estimating recombination rates and linkage between sampled markers and target loci. As sequencing costs decrease, low‐coverage whole‐genome sequencing of pooled or indexed‐individual samples is commonly utilized to identify loci associated with phenotypes or environmental axes in non‐model organisms. There are, however, relatively few publicly available bioinformatic pipelines designed explicitly to analyse these types of data, and fewer still that process the raw sequencing data, provide useful metrics of quality control and then execute analyses. Here, we present an updated version of a bioinformatics pipeline calledPoolParty2that can effectively handle either pooled or indexed DNA samples and includes new features to improve computational efficiency. Using simulated data, we demonstrate the ability of our pipeline to recover segregating variants, estimate their allele frequencies accurately, and identify genomic regions harbouring loci under selection. Based on the simulated data set, we benchmark the efficacy of our pipeline with another bioinformatic suite,angsd, and illustrate the compatibility and complementarity of these suites usingangsdto generate genotype likelihoods as input for identifying linkage outlier regions using alignment files and variants provided byPoolParty2. Finally, we apply our updated pipeline to an empirical dataset of low‐coverage whole genomic data from population samples of Columbia River steelhead trout (Oncorhynchus mykiss), results from which demonstrate the genomic impacts of decades of artificial selection in a prominent hatchery stock. Thus, we not only demonstrate the utility ofPoolParty2for genomic studies that combine sequencing data from multiple individuals, but also illustrate how it compliments other bioinformatics resources such asangsd.  more » « less
Award ID(s):
1757324
PAR ID:
10495578
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
Wiley
Date Published:
Journal Name:
Molecular Ecology Resources
ISSN:
1755-098X
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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