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1. The long and short of the S ‐locus in Turnera (Passifloraceae)

   https://doi.org/10.1111/nph.15970  

   Shore, Joel S.; Hamam, Hasan J.; Chafe, Paul D. J.; Labonne, Jonathan D. J.; Henning, Paige M.; McCubbin, Andrew G. (July 2019, New Phytologist)

   Summary Distyly is an intriguing floral adaptation that increases pollen transfer precision and restricts inbreeding. It has been a model system in evolutionary biology since Darwin. Although theS‐locus determines the long‐ and short‐styled morphs, the genes were unknown inTurnera. We have now identified these genes.We used deletion mapping to identify, and then sequence,BACclones and genome scaffolds to constructS/shaplotypes. We investigated candidate gene expression, hemizygosity, and used mutants, to explore gene function.Thes‐haplotype possessed 21 genes collinear with a region of chromosome 7 of grape. TheS‐haplotype possessed three additional genes and two inversions.TsSPH1was expressed in filaments and anthers,TsYUC6in anthers andTsBAHDin pistils. Long‐homostyle mutants did not possessTsBAHDand a short‐homostyle mutant did not expressTsSPH1.Three hemizygous genes appear to determine S‐morph characteristics inT. subulata. Hemizygosity is common to all distylous species investigated, yet the genes differ. The pistil candidate gene,TsBAHD, differs from that ofPrimula, but both may inactivate brassinosteroids causing short styles.TsYUC6is involved in auxin synthesis and likely determines pollen characteristics.TsSPH1is likely involved in filament elongation. We propose an incompatibility mechanism involvingTsYUC6andTsBAHD. 

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2. The R -Process Alliance: Analysis of limited- r stars

   https://doi.org/10.1051/0004-6361/202449376  

   Xylakis-Dornbusch, T; Hansen, T T; Beers, T C; Christlieb, N; Ezzeddine, R; Frebel, A; Holmbeck, E; Placco, V M; Roederer, I U; Sakari, C M; et al (August 2024, Astronomy & Astrophysics)

   Context.In recent years, theR-Process Alliance (RPA) has conducted a successful search for stars that are enhanced in elements produced by the rapid neutron-capture (r-)process. In particular, the RPA has uncovered a number of stars that are strongly enriched in lightr-process elements, such as Sr, Y, and Zr. These so-called limited-rstars were investigated to explore the astrophysical production site(s) of these elements. Aims.We investigate the possible formation sites for light neutron-capture elements by deriving detailed abundances for neutron-capture elements from high-resolution spectra with a high signal-to-noise ratio of three limited-rstars. Methods.We conducted a kinematic analysis and a 1D local thermodynamic equilibrium spectroscopic abundance analysis of three stars. Furthermore, we calculated the lanthanide mass fraction (X_La) of our stars and of limited-rstars from the literature. Results.We found that the abundance pattern of neutron-capture elements of limited-rstars behaves differently depending on their [Ba/Eu] ratios, and we suggest that this should be taken into account in future investigations of their abundances. Furthermore, we found that theX_Laof limited-rstars is lower than that of the kilonova AT2017gfo. The latter seems to be in the transition zone between limited-rX_Laand that ofr-I andr-II stars. Finally, we found that unliker-I andr-II stars, the current sample of limited-rstars is largely born in the Galaxy and is not accreted. 

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3. The phenotypic spectrum of PTCD3 deficiency

   https://doi.org/10.1002/jmd2.12424  

   Lace, Baiba; Faqeih, Eissa; Kaya, Namik; Krumina, Zita; Mayr, Johannes_A; Micule, Ieva; Wright, Nathan_Thompson; Achleitner, Melanie_T; AlQudairy, Hanan; Pajusalu, Sander; et al (May 2024, JIMD Reports)

   Abstract ThePTCD3gene product (protein PTCD3 or MRPS39) forms the entry channel of the mitochondrial small ribosomal subunit and binds to single‐stranded mRNA. Here, we expand on the clinical manifestations ofPTCD3pathogenic variants by describing an early‐onset patient with Leigh‐like syndrome and two patients with milder form of disease, with combined oxidative phosphorylation deficiency. A 34‐year‐old male and his 33‐year‐old sister both have horizontal nystagmus, pronounced rough tremor, truncal ataxia, dysmetria, spasticity and hyperreflexia. The basal respiration rate decreased significantly for the male patient and his mother (p < 0.0001) compared to the controls. The whole genome sequencing analysis revealed two heterozygous variants in thePTCD3: c.1182T>A, p.(Tyr394Ter) and c.805C>T, p.(His269Tyr). Tyr394Ter variant ablates the C‐terminal half of the protein, including a significant portion of the central fold. In silico modelling for the variant His269Tyr shows that the inclusion of the slightly larger tyrosine sidechain is well tolerated, with no significant change in either the position or the movement of the surrounding area. The third case is a 9‐year‐old boy, who has a global developmental delay, central hypotonia, hyperreflexia and abnormal MRI.PTCD3pathogenic variant c.538+4A>G was identified by whole exome sequencing. To test the variant's effect on splicing, an RT‐PCR experiment was performed, which revealed skipping of an out‐of‐frame exon 7. 

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4. Epitaxial growth of the first two members of the Ba n +1In n O2.5 n +1 Ruddlesden–Popper homologous series

   https://doi.org/10.1116/6.0002205  

   Hensling, Felix_V_E; Smeaton, Michelle_A; Show, Veronica; Azizie, Kathy; Barone, Matthew_R; Kourkoutis, Lena_F; Schlom, Darrell_G (November 2022, Journal of Vacuum Science & Technology A)

   We demonstrate the epitaxial growth of the first two members, and the n=∞ member of the homologous Ruddlesden–Popper series of Ban+1InnO2.5n+1 of which the n=1 member was previously unknown. The films were grown by suboxide molecular-beam epitaxy where the indium is provided by a molecular beam of indium-suboxide [In2O (g)]. To facilitate ex situ characterization of the highly hygroscopic barium indate films, a capping layer of amorphous SiO2 was deposited prior to air exposure. The structural quality of the films was assessed by x-ray diffraction, reflective high-energy electron diffraction, and scanning transmission electron microscopy. 

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5. Paludicola gen. nov. and Revision of the Species Formerly in Batrachospermum Section  Turfosa (Batrachospermales, Rhodophyta)

   https://doi.org/10.1111/jpy.13001  

   Vis, Morgan L.; Lee, Janina; Eloranta, Pertti; Chapuis, Iara S.; Lam, Daryl W.; Necchi, Jr., Orlando; Oliveira, ed., M. C. (May 2020, Journal of Phycology)

   Since the first phylogenetic study of the order Batrachospermales,Batrachospermumwas shown to be paraphyletic. Subsequently, sections of the genus have been methodically investigated usingDNAsequences and morphology in order to propose new genera and delineate species.BatrachospermumsectionTurfosais the last section with multiple species yet to be examined. New sequence data of specimens from Europe and the United States were combined with the sparse sequence data already available. Phylogenetic analyses usingrbcL andCOI‐5P sequences showed this section to be a well‐supported clade, distinct fromBatrachospermumsectionBatrachospermumand its segregate genera. Section Turfosais raised to the generic rank asPaludicolagen. nov. Substantial genetic variation within the genus was discovered and 12 species are recognized based onDNAsequence data as well as morphological characters and geographic distribution. The following morphological characters were applied to distinguish species: branching pattern (pseudodichotomous or irregular), whorl size (reduced or well developed), primary fascicles (curved or straight), spermatangia origin (primary or secondary fascicles), and carposporophyte arrangement (loose or dense). Previously published species were transferred to the new genus:P. turfosa,P. keratophyta,P. orthosticha,P. phangiae,andP. periploca. Seven new species are proposed as follows:P. groenbladiifrom Europe;P. communis,P. johnhallii, andP. leafensisfrom North America; andP. aquanigra,P. diamantinensis, andP. turfosiformisfrom Brazil. In addition, three unsequenced species in the section,P. bakarensis,P. gombakensis, andP. tapirensis, were transferred to the new genus. 

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