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1. A mathematical model for Vibrio-phage interactions

   https://doi.org/10.3934/mbe.2021137  

   Botelho, Christopher; Kong, Jude Dzevela; Lucien, Mentor Ali; Shuai, Zhisheng; Wang, Hao (January 2021, Mathematical Biosciences and Engineering)

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2. Vibrio floridensis sp. nov., a novel species closely related to the human pathogen Vibrio vulnificus isolated from a cyanobacterial bloom

   https://doi.org/10.1099/ijsem.0.005675  

   Grant, Trudy-Ann; Jayakumar, Jane M.; López-Pérez, Mario; Almagro-Moreno, Salvador (February 2023, International Journal of Systematic and Evolutionary Microbiology)

   A Gram-stain-negative, rod-shaped bacterial strain, designatedVibrio floridensisIRLE0018 (=NRRL B-65642=NCTC 14661), was isolated from a cyanobacterial bloom along the Indian River Lagoon (IRL), a large and highly biodiverse estuary in eastern Florida (USA). The results of phylogenetic, biochemical, and phenotypic analyses indicate that this isolate is distinct from species of the genusVibriowith validly published names and is the closest relative to the emergent human pathogen,Vibrio vulnificus. Here, we present the complete genome sequence ofV. floridensisstrain IRLE0018 (4 535 135 bp). On the basis of the established average nucleotide identity (ANI) values for the determination of different species (ANI <95 %), strain IRLE0018, with an ANI of approximately 92 % compared with its closest relative,V. vulnificus, represents a novel species within the genusVibrio. To our knowledge, this represents the first time this species has been described. The results of genomic analyses ofV. floridensisIRLE0018 indicate the presence of antibiotic resistance genes and several known virulence factors, however, its pathogenicity profile (e.g. survival in serum, phagocytosis avoidance) reveals limited virulence potential of this species in contrast toV. vulnificus. 

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3. Quorum sensing controls Vibrio cholerae multicellular aggregate formation

   https://doi.org/10.7554/eLife.42057  

   Jemielita, Matthew; Wingreen, Ned S; Bassler, Bonnie L (December 2018, eLife)

   Bacteria communicate and collectively regulate gene expression using a process called quorum sensing (QS). QS relies on group-wide responses to signal molecules called autoinducers. Here, we show that QS activates a new program of multicellularity in Vibrio cholerae. This program, which we term aggregation, is distinct from the canonical surface-biofilm formation program, which QS represses. Aggregation is induced by autoinducers, occurs rapidly in cell suspensions, and does not require cell-division, features strikingly dissimilar from those characteristic of V. cholerae biofilm formation. Extracellular DNA limits aggregate size, but is not sufficient to drive aggregation. A mutagenesis screen identifies genes required for aggregate formation, revealing proteins involved in V. cholerae intestinal colonization, stress response, and a protein that distinguishes the current V. cholerae pandemic strain from earlier pandemic strains. We suggest that QS-controlled aggregate formation is important for V. cholerae to successfully transit between the marine niche and the human host. 

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4. Structural determinants of Vibrio cholerae FeoB nucleotide promiscuity

   https://doi.org/10.1016/j.jbc.2024.107663  

   Lee, Mark; Magante, Kate; Gómez-Garzón, Camilo; Payne, Shelley M; Smith, Aaron T (September 2024, Journal of Biological Chemistry)
5. A role for Vibrio vulnificus PecS during hypoxia

   https://doi.org/10.1038/s41598-019-39095-4  

   Bhattacharyya, Nabanita; Lemon, Tiffany L.; Grove, Anne (February 2019, Scientific Reports)

   Abstract The genusVibrioincludes serious human pathogens, and mollusks are a significant reservoir for species such asV.vulnificus.Vibriospecies encode PecS, a member of the multiple antibiotic resistance regulator (MarR) family of transcription factors;pecSis divergently oriented topecM, which encodes an efflux pump. We report here thatVibriospecies feature frequent duplications ofpecS-pecMgenes, suggesting evolutionary pressures to respond to distinct environmental situations. The singleV.vulnificusPecS binds two sites within thepecS-pecMintergenic region with K_d = 0.3 ± 0.1 nM, a binding that is attenuated by the ligands xanthine and urate, except when promoter DNA is saturated with PecS. A unique target is found in the intergenic region between genes encoding the nitric oxide sensing transcription factor, NsrR, andnod; thenod-encoded nitric oxide dioxygenase is important for preventing nitric oxide stress. Reporter gene assays show that PecS-mediated repression of gene expression can be relieved in presence of ligand. Since xanthine and urate are produced as part of the oxidative burst during host defenses and under molluscan hypoxia, we propose that these intermediates in the host purine degradation pathway function to promote bacterial survival during hypoxia and oxidative stress. 

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