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Butterflies and moths (Lepidoptera) comprise significant portions of the world’s natural history collections, but a standardized tissue preservation protocol for molecular research is largely lacking. Lepidoptera have traditionally been spread on mounting boards to display wing patterns and colors, which are often important for species identification. Many molecular phylogenetic studies have used legs from pinned specimens as the primary source for DNA in order to preserve a morphological voucher, but the amount of available tissue is often limited. Preserving an entire specimen in a cryogenic freezer is ideal for DNA preservation, but without an easily accessible voucher it can make specimen identification, verification, and morphological work difficult. Here we present a procedure that creates accessible and easily visualized “wing vouchers” of individual Lepidoptera specimens, and preserves the remainder of the insect in a cryogenic freezer for molecular research. Wings are preserved in protective holders so that both dorsal and ventral patterns and colors can be easily viewed without further damage. Our wing vouchering system has been implemented at the University of Maryland (AToL Lep Collection) and the University of Florida (Florida Museum of Natural History, McGuire Center of Lepidoptera and Biodiversity), which are among two of the largest Lepidoptera molecular collections in the world.
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Who is that?! Using Genetic and Morphological Data to Identify Cryptic African Tree Frog Specimens in Natural History Collections
Natural history collections are important resources for research and conservation. Unfortunately, sometimes certain taxa can be hard to identify to the species level or can be misidentified, impacting how those specimens and their accompanying data are used for future research and conservation. African tree frogs (Family Arthroleptidae, Genus Leptopelis) are a group of big-eyed tree frogs found across Sub-Saharan Africa. While they have morphological differences that can help tell members of the genus apart, when in the field species can look similar to each other which gives rise to them being misidentified or unidentified in collections. In particular, Leptopelis exhibit extensive intraspecific variation in coloration and pattern that makes identification based solely on morphology challenging. The goal of this project is to use DNA barcoding of the 16s mtDNA gene and morphological characteristics to update the identification of “Leptopelis sp.” specimens that are part of the California Academy of Science’s Herpetology collection to the species level. By comparing the genetic and morphological data collected to identified reference specimens in the Academy’s collection we can confirm species identification and update the Academy’s database. Having the opportunity to identify species in natural history collections using both genetic and morphological analysis helps us better understand what species are found in an area, and can reveal new localities and range expansions of poorly known species. These data serve as an important baseline for monitoring how species are adapting to global change and enable future research on these poorly known, charismatic frogs.
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- Award ID(s):
- 2243994
- PAR ID:
- 10502216
- Publisher / Repository:
- Society for the Advancement of Chicanos/Hispanics and Native Americans in Science - 2023 NDiSTEM
- Date Published:
- Format(s):
- Medium: X
- Location:
- Portland, OR
- Sponsoring Org:
- National Science Foundation
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