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  • Simplified Protocol to Demonstrate Gene Expression in Nicotiana benthamiana Using an Agrobacterium-Mediated Transient Assay
Title: Simplified Protocol to Demonstrate Gene Expression in Nicotiana benthamiana Using an Agrobacterium-Mediated Transient Assay
Agrobacterium-mediated transient gene expression in Nicotiana benthamiana is widely used to study gene function in plants. One dramatic phenotype that is frequently screened for is cell death. Here, we present a simplified protocol for Agrobacterium-mediated transient gene expression by infiltration. Compared with current methods, the novel protocol can be done without a centrifuge or spectrometer, thereby suitable for K-12 outreach programs as well as rapidly identifying genes that induce cell death.  more » « less
Award ID(s):
2114833
PAR ID:
10509383
Author(s) / Creator(s):
; ;
Editor(s):
Shamu, Caroline
Publisher / Repository:
Fanglian He
Date Published:
Journal Name:
BIO-PROTOCOL
Volume:
14
Issue:
10
ISSN:
2331-8325
Page Range / eLocation ID:
1-8
Subject(s) / Keyword(s):
Agrobacterium, Nicotiana benthamiana, Agroinfiltration, Cell death, Gene expression, Ion leakage
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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