An official website of the United States government
To combat climate change, one approach is to manufacture products from biomass-fungi composite materials instead of petroleum-based plastics. These products can be used in packaging, furniture, and construction industries. A 3D printing-based manufacturing method was developed for these biomass-fungi composite materials, eliminating the need for molds, and enabling customized product design. However, previous studies on the 3D printing-based method showed significant shrinkage of printed samples. In this paper, an approach is proposed to reduce the shrinkage by incorporating ionic crosslinking into biomass-fungi composite materials. This paper reports two sets of experiments regarding the effects of sodium alginate (SA) and calcium chloride (CaCl2) on fungal growth and fungal viability. The first set of experiments was conducted using Petri dishes with fungi isolated from colonized biomass-fungi material and different concentrations of SA and CaCl2. Fungal growth was measured by the circumference of fungal colonies. The results showed that concentrations of SA and CaCl2 had significant effects on fungal growth and no fungal growth was observed on Petri dishes with 15% CaCl2. Some of these Petri dishes were also observed under confocal microscopy. The results confirmed the differences obtained by measuring the circumference of fungal colonies. The second set of experiments was conducted using Petri dishes with biomass-fungi mixtures that were treated with different concentrations of SA and exposure times in a CaCl2 (crosslinking) solution. Fungal viability was measured by counting colony-forming units. The results showed that the addition of the SA solution and exposure times in the crosslinking solution had statistically significant effects on fungal viability. The 2SA solution was prepared by dissolving 2 g of SA in 100 mL of water, the 5SA solution was prepared by dissolving 5 g of SA in 100 mL of water, and the crosslinking solution was prepared by dissolving 5 g of CaCl2 in 100 mL of water. The results also showed that fungal viability was not too low in biomass-fungi mixtures that included 2SA solution and were exposed to the crosslinking solution for 1 min.
more »
« less
Studies on production of Anaheim pepper in greenhouse media supplemented with organic and inorganic nutrient sources, and water conservation
An experiment was conducted in the greenhouse to study growth, development, yield and water-use by Anahein pepper grown in a potting mixture supplemented with MiracleGro® (9:4:12) and milled alfalfa (3:1:2) as sources of nutrient. The study was comprised of 5 treatments, control (C), 3 gL-1 MiracleGro® (MG), 9 g alfalfa mill supplement (SA-1), 18 g (SA-2), and 27 g (SA-3), and replicated 6 times. Ten physiological and morphological parameters were used to evaluate growth, development and yield of the Anaheim pepper, and two parameters used to evaluate the water holding capacity of the potting mixture. The results indicate that the potting medium supplemented with alfalfa mill required significantly less water to support growth and development of the species. Also, growth, development and yield of Anaheim pepper was significantly higher in the organic supplements at SA-2 and SA-3.
more »
« less
- Award ID(s):
- 1914745
- PAR ID:
- 10517199
- Editor(s):
- Kumar, R
- Publisher / Repository:
- Society for Promotion of Horticulture (SPH)
- Date Published:
- Journal Name:
- Journal of Horticultural Sciences
- Volume:
- 18
- Issue:
- 2
- ISSN:
- 0973-354X
- Subject(s) / Keyword(s):
- Capsicum species, alfalfa, MiracleGro, growth and development, greenhouse
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
Water is a vital component for agricultural productivity; however, freshwater supplies are limited and are dwindling worldwide. Water for agriculture is an extreme issue for the southern region of Texas, where water supplies from reservoirs are used for municipal, industrial, and agricultural purposes. Due to intensive and prolonged intermittent droughts in south Texas, freshwater sources can deplete rapidly leaving growers on water restrictions. One potential solution of reducing the amount of water for crops is by applying less water than recommended crop evapotranspiration requires. Deficit irrigation (DI) is the practice of applying lower amounts of water than general crop requirements to increase water use efficiency for economic benefit. Deficit irrigation practice has been shown to be beneficial to some fruit and vegetable crops, but to a lesser extent in south Texas for mild heat pepper plant production. The purpose of this project was to analyze how watering jalapeño and serrano pepper plants at different levels of DI would impact plant growth and fruit yield in a greenhouse study. Deficit irrigation treatments were performed by irrigating pots at increasing the number of days between irrigation events (water application: 2, 4, 8, and 12 days) to create increasing water stress levels to plants. Plant growth and biomass data was collected to determine the impact of increasing deficit irrigation on plant shoot productivity. In both varieties, plant biomass steadily decreased as water application decreased. Serrano peppers grown at both 4d and 2d between water application events produced identical yields, however, increased water stress immediately impacted jalapeño peppers with lower yield. The encouraging results from serrano peppers suggest a potential economic benefit for deficit irrigation water use practices applied to this pepper variety.more » « less
-
Water is a vital component for agricultural productivity; however, freshwater supplies are limited and are dwindling worldwide. Water for agriculture is an extreme issue for the southern region of Texas, where water supplies from reservoirs are used for municipal, industrial, and agricultural purposes. Due to intensive and prolonged intermittent droughts in south Texas, freshwater sources can deplete rapidly leaving growers on water restrictions. One potential solution of reducing the amount of water for crops is by applying less water than recommended crop evapotranspiration requires. Deficit irrigation (DI) is the practice of applying lower amounts of water than general crop requirements to increase water use efficiency for economic benefit. Deficit irrigation practice has been shown to be beneficial to some fruit and vegetable crops, but to a lesser extent in south Texas for mild heat pepper plant production. The purpose of this project was to analyze how watering jalapeño and serrano pepper plants at different levels of DI would impact plant growth and fruit yield in a greenhouse study. Deficit irrigation treatments were performed by irrigating pots at increasing the number of days between irrigation events (water application: 2, 4, 8, and 12 days) to create increasing water stress levels to plants. Plant growth and biomass data was collected to determine the impact of increasing deficit irrigation on plant shoot productivity. In both varieties, plant biomass steadily decreased as water application decreased. Serrano peppers grown at both 4d and 2d between water application events produced identical yields, however, increased water stress immediately impacted jalapeño peppers with lower yield. The encouraging results from serrano peppers suggest a potential economic benefit for deficit irrigation water use practices applied to this pepper variety.more » « less
-
At two sites in the North Central USA (Michigan (KBS) and Wisconsin (ARL)), we evaluated the effect of N fertilization on the yield and quality of five perennial bioenergy feedstock cropping systems: (1) switchgrass (Panicum virgatum L.), (2) giant miscanthus (Miscanthus × giganteus), (3) a native grass mixture (5 species), (4) an early successional field (volunteer herbaceous species), and (5) a restored prairie (18 species). In a randomized complete block design with 5 replicates and 2 split plots, N was applied at 0 and 56 kg ha−1 to split plots for each cropping system from 2010 to 2016. No yield response to N was detected in switchgrass at either location in any year. Giant miscanthus exhibited a positive yield response to N at both sites (11% at KBS and 83% at ARL). Nitrogen fertilizer addition significantly reduced glucose (KBS 12.9 and 13.8 g kg−1 year−1, ARL 11.2 and 9.7 g kg−1 year−1) in the native grass mix and restored prairie systems respectively. Nitrogen fertilizer also reduced xylose at KBS in the switchgrasss, native grass mix, and restored prairie (4.9, 7.5, and 5.0 g kg−1 year−1). At ARL, N fertilization reduced xylose levels in switchgrass, giant miscanthus, and restored prairie (7.4, 6.8, and 6.2 g kg−1 year−1) and increased xylose levels in the early successional system (5.0 g kg−1 year−1).more » « less
-
A multifunctional polysaccharide-based hydrogel was studied as an additive for enhancing microalgae growth. The hydrogel was fabricated by physically and chemically crosslinking renewable ingredients of carboxymethyl cellulose (CMC), arrowroot starch, and activated biochar modified with iron using a bio-crosslinker of oxidized sucrose and a plasticizer of glycerol. The optimum formula for the hydrogel with a high swelling ratio, BET surface area, and electrical conductivity was found to include 1 g starch, 3 g CMC, 1.5 g biochar, 15 mL oxidized sucrose, and 1.5 mL glycerol in 200 mL deionized water. The algal yield and cell concentration after 14 days of growth in a Bold basal medium with an optimum concentration of 2.5 g hydrogel/L increased by 65.7 % and 92.2 %, respectively, compared to those of the control without the hydrogel. However, if the hydrogel concentration in the culture increased to 12.5 g/L, the algal yield was decreased by 67.8 % compared to the control due to oxidative injury. The hydrogel additive could significantly increase the nitrogen but decrease the carbon, hydrogen, and sulfur contents of the microalgae. The algal yield with 2.5 g/L hydrogel additive improved by 13.9 % compared to the algal yield with the same amounts of individual non–crosslinked hydrogel ingredients.more » « less
- Free Publicly Accessible Full Text
-
Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.24154/jhs.v18i2.2005
