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Abstract Dormant propagules can provide a rapid colonization source for temporary aquatic habitats and set the trajectory for community dynamics, yet the egg banks of stormwater management systems have received little attention. We asked which species hatched from the sediment of drainage ditches in Champaign County, IL, and found bdelloid rotifers and ostracods (Heterocypris incongruens) to be the most common taxa. These sites also are colonized by mosquitoes, and we established laboratory experiments to examine interspecific interactions between common co‐occurring taxa.Culex restuanslarvae were reared in the presence or absence ofH. incongruensat two intra‐ and interspecific densities (20 or 40 total individuals) and their survivorship to adulthood, development time to adulthood, adult body size, and sex ratio were determined. Survival forCx. restuanswas significantly lower at high larval density than at low larval density in both treatments.Culex restuanslarvae reared in the presence ofH. incongruenshad a shorter development time to adulthood and emerged as larger adults compared to those reared in the absence ofH. incongruens. The sex ratios in theH. incongruenstreatments were female‐biased whereas those in theCulex‐only treatments were male‐biased. These differences may have epidemiological implications, as only female mosquitoes serve as disease vectors. Our results emphasize the importance of understanding interspecific interactions in influencing larval mosquito development traits.
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Rearing and Maintaining a Culex Colony in the Laboratory
After overcoming the significant obstacle of getting adultCulexmosquitoes to reproduce and blood feed in the laboratory, maintaining a laboratory colony is much more achievable. However, great care and attention to detail are still required to ensure that the larvae have adequate food without being overwhelmed by bacterial growth. Additionally, achieving the appropriate densities of larvae and pupae is essential, as overcrowding delays development, prevents pupae from successfully emerging as adults, and/or reduces adult fecundity and alters sex ratios. Finally, adult mosquitoes should have constant access to H2O and nearly constant access to sugar sources to ensure that both males and females have adequate nutrition and can produce the maximum number of offspring. Here, we describe our methods for maintaining the Buckeye strain ofCulex pipiensand how other researchers might modify them to suit their specific needs.
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- Award ID(s):
- 1944324
- PAR ID:
- 10521081
- Publisher / Repository:
- Cold Spring Harbor Protocols
- Date Published:
- Journal Name:
- Cold Spring Harbor Protocols
- Volume:
- 2023
- Issue:
- 8
- ISSN:
- 1940-3402
- Page Range / eLocation ID:
- pdb.prot108080
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
-
Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1101/pdb.prot108080
