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Title: Points to Consider When Establishing and Rearing Culex Mosquitoes in the Laboratory
Culexmosquitoes transmit several pathogens to humans and animals, including viruses that cause West Nile fever and St. Louis encephalitis and filarial nematodes that cause canine heartworm and elephantiasis. Additionally, these mosquitoes have a cosmopolitan distribution and provide interesting models for understanding population genetics, overwintering dormancy, disease transmission, and other important and ecological questions. However, unlikeAedesmosquitoes that produce eggs that can be stored for weeks at a time, no obvious “stopping” point exists in the development ofCulexmosquitoes. Therefore, these mosquitoes require nearly continuous care and attention. Here, we describe some general considerations when rearing laboratory colonies ofCulexmosquitoes. We highlight different methods so that readers may choose what works best for their experimental needs and laboratory infrastructure. We hope that this information will enable additional scientists to conduct laboratory research on these important disease vectors.  more » « less
Award ID(s):
1944324
PAR ID:
10521083
Author(s) / Creator(s):
; ;
Publisher / Repository:
Cold Spring Harbor Protocols
Date Published:
Journal Name:
Cold Spring Harbor Protocols
Volume:
2023
Issue:
8
ISSN:
1940-3402
Page Range / eLocation ID:
pdb.top107823
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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