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Title: De Novo Long-Read Genome Assembly and Annotation of the Luna Moth ( Actias luna ) Fully Resolves Repeat-Rich Silk Genes
Abstract We present the first long-read de novo assembly and annotation of the luna moth (Actias luna) and provide the full characterization of heavy chain fibroin (h-fibroin), a long and highly repetitive gene (>20 kb) essential in silk fiber production. There are >160,000 described species of moths and butterflies (Lepidoptera), but only within the last 5 years have we begun to recover high-quality annotated whole genomes across the order that capture h-fibroin. Using PacBio HiFi reads, we produce the first high-quality long-read reference genome for this species. The assembled genome has a length of 532 Mb, a contig N50 of 16.8 Mb, an L50 of 14 contigs, and 99.4% completeness (BUSCO). Our annotation using Bombyx mori protein and A. luna RNAseq evidence captured a total of 20,866 genes at 98.9% completeness with 10,267 functionally annotated proteins and a full-length h-fibroin annotation of 2,679 amino acid residues.  more » « less
Award ID(s):
2217159 2217155
PAR ID:
10525004
Author(s) / Creator(s):
; ; ; ; ; ;
Publisher / Repository:
Oxford University Press
Date Published:
Journal Name:
Genome Biology and Evolution
Volume:
16
Issue:
7
ISSN:
1759-6653
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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