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Title: A single cell RNA sequencing resource for early sea urchin development
ABSTRACT Identifying cell states during development from their mRNA profiles provides insight into their gene regulatory network. Here, we leverage the sea urchin embryo for its well-established gene regulatory network to interrogate the embryo using single cell RNA sequencing. We tested eight developmental stages in Strongylocentrotus purpuratus, from the eight-cell stage to late in gastrulation. We used these datasets to parse out 22 major cell states of the embryo, focusing on key transition stages for cell type specification of each germ layer. Subclustering of these major embryonic domains revealed over 50 cell states with distinct transcript profiles. Furthermore, we identified the transcript profile of two cell states expressing germ cell factors, one we conclude represents the primordial germ cells and the other state is transiently present during gastrulation. We hypothesize that these cells of the Veg2 tier of the early embryo represent a lineage that converts to the germ line when the primordial germ cells are deleted. This broad resource will hopefully enable the community to identify other cell states and genes of interest to expose the underpinning of developmental mechanisms.  more » « less
Award ID(s):
1923445
PAR ID:
10528196
Author(s) / Creator(s):
; ;
Publisher / Repository:
Company of Biologists
Date Published:
Journal Name:
Development
Volume:
147
Issue:
17
ISSN:
0950-1991
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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