Analyte migration order is a major aspect in all migration-based analytical separations methods. Presented here is the manipulation of the migration order of microparticles in an insulator-based electrokinetic separation. Three distinct particle mixtures were studied: a binary mixture of particles with similar electrical charge and different sizes, and two tertiary mixtures of particles of distinct sizes. Each one of the particle mixtures was separated twice, the first separation was performed under low voltage (linear electrokinetic regime) and the second separation was performed under high voltage (nonlinear electrokinetic regime). Linear electrophoresis, which discriminates particles by charge, is the dominant electrokinetic effect in the linear regime; while nonlinear electrophoresis, which discriminates particles by size and shape, is the dominant electrokinetic effect in the nonlinear regime. The separation results obtained with the three particle mixtures illustrated that particle elution order can be changed by switching from the linear electrokinetic regime to the nonlinear electrokinetic regime. Also, in all cases, better separation performances in terms of separation resolution (Rs) were obtained by employing the nonlinear electrokinetic regime allowing nonlinear electrophoresis to be the discriminatory electrokinetic mechanism. These findings could be applied to analyze complex samples containing bioparticles of interest within the micron size range. This is the first report where particle elution order is altered in an iEK system.
This content will become publicly available on July 17, 2025
Nonlinear electrokinetic phenomena offer label-free, portable, and robust approaches for particle and cell assessment, including selective enrichment, separation, sorting, and characterization. The field of electrokinetics has evolved substantially since the first separation reports by Arne Tiselius in the 1930s. The last century witnessed major advances in the understanding of the weak-field theory, which supported developments in the use of linear electrophoresis and its adoption as a routine analytical technique. More recently, an improved understanding of the strong-field theory enabled the development of nonlinear electrokinetic techniques such as electrorotation, dielectrophoresis, and nonlinear electrophoresis. This review discusses the operating principles and recent applications of these three nonlinear electrokinetic phenomena for the analysis and manipulation of particles and cells and provides an overview of some of the latest developments in the field of nonlinear electrokinetics.
more » « less- PAR ID:
- 10528778
- Publisher / Repository:
- Annual Reviews
- Date Published:
- Journal Name:
- Annual Review of Analytical Chemistry
- Volume:
- 17
- Issue:
- 1
- ISSN:
- 1936-1327
- Page Range / eLocation ID:
- 243 to 264
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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