A Gram-stain-negative, strictly anaerobic, non-motile, rod-shaped bacterium, designated SFB93T, was isolated from the intertidal sediments of South San Francisco Bay, located near Palo Alto, CA, USA. SFB93Twas capable of acetylenotrophic and diazotrophic growth, grew at 22–37 °C, pH 6.3–8.5 and in the presence of 10–45 g l−1NaCl. Phylogenetic analyses based on 16S rRNA gene sequencing showed that SFB93Trepresented a member of the genus
The evolution of a novel trait can profoundly change an organism’s effects on its environment, which can in turn affect the further evolution of that organism and any coexisting organisms. We examine these effects and feedbacks following the evolution of a novel function in the Long-Term Evolution Experiment (LTEE) with
- Award ID(s):
- 1951307
- PAR ID:
- 10532397
- Publisher / Repository:
- Microbiology
- Date Published:
- Journal Name:
- Microbiology
- Volume:
- 169
- Issue:
- 8
- ISSN:
- 1350-0872
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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with highest 16S rRNA gene sequence similarities toSyntrophotalea DSM 3246T(96.6 %),Syntrophotalea acetylenica DSM 2380T(96.5 %), andSyntrophotalea carbinolica DSM 2394T(96.7 %). Genome sequencing revealed a genome size of 3.22 Mbp and a DNA G+C content of 53.4 %. SFB93Thad low genome-wide average nucleotide identity (81–87.5 %) and <70 % digital DNA–DNA hybridization value with other members of the genusSyntrophotalea venetiana . The phylogenetic position of SFB93Twithin the familySyntrophotalea and as a novel member of the genusSyntrophotaleaceae was confirmed via phylogenetic reconstruction based on concatenated alignments of 92 bacterial core genes. On the basis of the results of phenotypic, genotypic and phylogenetic analyses, a novel species,Syntrophotalea Syntrophotalea acetylenivorans sp. nov., is proposed, with SFB93T(=DSM 106009T=JCM 33327T=ATCC TSD-118T) as the type strain. -
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Vibrio floridensis IRLE0018 (=NRRL B-65642=NCTC 14661), was isolated from a cyanobacterial bloom along the Indian River Lagoon (IRL), a large and highly biodiverse estuary in eastern Florida (USA). The results of phylogenetic, biochemical, and phenotypic analyses indicate that this isolate is distinct from species of the genus with validly published names and is the closest relative to the emergent human pathogen,Vibrio . Here, we present the complete genome sequence ofVibrio vulnificus V. floridensis strain IRLE0018 (4 535 135 bp). On the basis of the established average nucleotide identity (ANI) values for the determination of different species (ANI <95 %), strain IRLE0018, with an ANI of approximately 92 % compared with its closest relative, , represents a novel species within the genusV. vulnificus . To our knowledge, this represents the first time this species has been described. The results of genomic analyses ofVibrio V. floridensis IRLE0018 indicate the presence of antibiotic resistance genes and several known virulence factors, however, its pathogenicity profile (e.g. survival in serum, phagocytosis avoidance) reveals limited virulence potential of this species in contrast to .V. vulnificus -
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