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Title: New alleles of nlp-2, nlp-22, and nlp-23 demonstrate that they are dispensable for stress-induced sleep in C. elegans
Sleep is ancient and genetically conserved across phylogeny. Neuropeptide signaling plays a fundamental role in the regulation of sleep for mammals, fish, and invertebrates like Caenorhabditis elegans. Developmentally timed-sleep and stress-induced sleep of C. elegans are controlled by distinct and overlapping neuropeptide pathways. The RPamide neuropeptides nlp-2, nlp-22, and nlp-23, play antagonistic roles during the regulation of developmentally-timed sleep, however, their role in stress-induced sleep has not been explored. These genes are linked on the X chromosome, which has made genetic analyses challenging. Here we used CRISPR to generate new alleles of nlp-22 and nlp-23, nlp-22;nlp-23 double mutants, and nlp-2;nlp-22;nlp-23 triple mutants. Confirming previous studies, we find that nlp-22 is required for developmentally-timed sleep, and show that nlp-23 is also required. However, all three genes are dispensable for stress-induced sleep.  more » « less
Award ID(s):
1845020
PAR ID:
10533959
Author(s) / Creator(s):
; ;
Publisher / Repository:
microPublication Biology
Date Published:
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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