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1. Emerging investigator series: emerging biotechnologies in wastewater treatment: from biomolecular engineering to multiscale integration

   https://doi.org/10.1039/D0EW00393J  

   Zhang, Yixin; Hsu, Huan-Hsuan; Wheeler, John J.; Tang, Sishuang; Jiang, Xiaocheng (July 2020, Environmental Science: Water Research & Technology)

   null (Ed.)

   Biological wastewater treatment is the process in which contaminants can be removed or degraded by various microorganisms to eliminate the negative impact on environment and human health. Given the fact that traditional physical and chemical purification methods are high-cost, unsustainable and unspecific, biotreatment is playing an increasingly important role in the wastewater treatment field. The effective implementation of biotreatment strategy relies strongly on the intrinsic degradation capability of the microorganisms as well as their interaction with pollutants. In this review, we will focus on recent technological advances in engineering and improving biotreatment at both biocatalyst and bioreactor levels. Specifically, we will discuss the progress in synthetic biology for enhancing biosorption and biotransformation, and the challenges in applying engineered microorganisms on contaminated sites. We will further review the latest developments in bioreactor design, particularly the prospects of additive manufacturing/bioprinting to further optimize the mass transport inside bioreactors through complex 3-D structures and flexible material selections. These research efforts are redefining the frontier of biotreatment, and opening up new opportunities for cost-effective, efficient, and sustainable wastewater treatment. 

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2. Metabolic Differentiation of Co-occurring Accumulibacter Clades Revealed through Genome-Resolved Metatranscriptomics

   https://doi.org/10.1128/mSystems.00474-21  

   McDaniel, Elizabeth A.; Moya-Flores, Francisco; Keene Beach, Natalie; Camejo, Pamela Y.; Oyserman, Ben O.; Kizaric, Matthew; Khor, Eng Hoe; Noguera, Daniel R.; McMahon, Katherine D. (July 2021, mSystems)

   Hug, Laura A. (Ed.)

   ABSTRACT Natural microbial communities consist of closely related taxa that may exhibit phenotypic differences and inhabit distinct niches. However, connecting genetic diversity to ecological properties remains a challenge in microbial ecology due to the lack of pure cultures across the microbial tree of life. “ Candidatus Accumulibacter phosphatis” (Accumulibacter) is a polyphosphate-accumulating organism that contributes to the enhanced biological phosphorus removal (EBPR) biotechnological process for removing excess phosphorus from wastewater and preventing eutrophication from downstream receiving waters. Distinct Accumulibacter clades often coexist in full-scale wastewater treatment plants and laboratory-scale enrichment bioreactors and have been hypothesized to inhabit distinct ecological niches. However, since individual strains of the Accumulibacter lineage have not been isolated in pure culture to date, these predictions have been made solely on genome-based comparisons and enrichments with varying strain compositions. Here, we used genome-resolved metagenomics and metatranscriptomics to explore the activity of coexisting Accumulibacter strains in an engineered bioreactor environment. We obtained four high-quality genomes of Accumulibacter strains that were present in the bioreactor ecosystem, one of which is a completely contiguous draft genome scaffolded with long Nanopore reads. We identified core and accessory genes to investigate how gene expression patterns differed among the dominating strains. Using this approach, we were able to identify putative pathways and functions that may confer distinct functions to Accumulibacter strains and provide key functional insights into this biotechnologically significant microbial lineage. IMPORTANCE “ Candidatus Accumulibacter phosphatis” is a model polyphosphate-accumulating organism that has been studied using genome-resolved metagenomics, metatranscriptomics, and metaproteomics to understand the EBPR process. Within the Accumulibacter lineage, several similar but diverging clades are defined by the shared sequence identity of the polyphosphate kinase ( ppk1 ) locus. These clades are predicted to have key functional differences in acetate uptake rates, phage defense mechanisms, and nitrogen-cycling capabilities. However, such hypotheses have largely been made based on gene content comparisons of sequenced Accumulibacter genomes, some of which were obtained from different systems. Here, we performed time series genome-resolved metatranscriptomics to explore gene expression patterns of coexisting Accumulibacter clades in the same bioreactor ecosystem. Our work provides an approach for elucidating ecologically relevant functions based on gene expression patterns between closely related microbial populations. 

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3. Modification of a dual‐chamber denitrification bioreactor with a surface water pumping system

   https://doi.org/10.1002/agg2.20399  

   Hartfiel, Lindsey M.; Craig, Andrew J.; Soupir, Michelle L. (September 2023, Agrosystems, Geosciences & Environment)

   Core Ideas A subsurface drainage‐fed bioreactor was retrofitted with a supplemental surface water pumping system. Design criteria of the pumping system are presented along with challenges and future recommendations. Pumped bioreactor systems show promise for the treatment of alternative nitrate‐laden sources of water. Pumped bioreactors have the potential to remove nitrate beyond the typical drainage season. 

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4. Comparison of metagenomes from fermentation of various agroindustrial residues suggests a common model of community organization

   https://doi.org/10.3389/fbioe.2023.1197175  

   Myers, Kevin S.; Ingle, Abel T.; Walters, Kevin A.; Fortney, Nathaniel W.; Scarborough, Matthew J.; Donohue, Timothy J.; Noguera, Daniel R. (May 2023, Frontiers in Bioengineering and Biotechnology)

   Strik, David (Ed.)

   The liquid residue resulting from various agroindustrial processes is both rich in organic material and an attractive source to produce a variety of chemicals. Using microbial communities to produce chemicals from these liquid residues is an active area of research, but it is unclear how to deploy microbial communities to produce specific products from the different agroindustrial residues. To address this, we fed anaerobic bioreactors one of several agroindustrial residues (carbohydrate-rich lignocellulosic fermentation conversion residue, xylose, dairy manure hydrolysate, ultra-filtered milk permeate, and thin stillage from a starch bioethanol plant) and inoculated them with a microbial community from an acid-phase digester operated at the wastewater treatment plant in Madison, WI, United States. The bioreactors were monitored over a period of months and sampled to assess microbial community composition and extracellular fermentation products. We obtained metagenome assembled genomes (MAGs) from the microbial communities in each bioreactor and performed comparative genomic analyses to identify common microorganisms, as well as any community members that were unique to each reactor. Collectively, we obtained a dataset of 217 non-redundant MAGs from these bioreactors. This metagenome assembled genome dataset was used to evaluate whether a specific microbial ecology model in which medium chain fatty acids (MCFAs) are simultaneously produced from intermediate products (e.g., lactic acid) and carbohydrates could be applicable to all fermentation systems, regardless of the feedstock. MAGs were classified using a multiclass classification machine learning algorithm into three groups, organisms fermenting the carbohydrates to intermediate products, organisms utilizing the intermediate products to produce MCFAs, and organisms producing MCFAs directly from carbohydrates. This analysis revealed common biological functions among the microbial communities in different bioreactors, and although different microorganisms were enriched depending on the agroindustrial residue tested, the results supported the conclusion that the microbial ecology model tested was appropriate to explain the MCFA production potential from all agricultural residues. 

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5. Filtration Methods for Microplastic Removal in Wastewater Streams — A Review

   https://doi.org/10.1142/S0129156423500192  

   Salahuddin, U.; Sun, J.; Zhu, C.; Gao, P. (December 2023, International Journal of High Speed Electronics and Systems)

   Microplastics are commonly recognized as environmental and biotic contaminants. The prevalent presence of microplastics in aquatic settings raises concerns about plastic pollution. Therefore, it is critical to develop methods that can eliminate these microplastics with low cost and high effectiveness. This review concisely provides an overview of various methods and technologies for removing microplastics from wastewater and marine environments. Dynamic membranes and membrane bioreactors are effective in removing microplastics from wastewater. Chemical methods such as coagulation and sedimentation, electrocoagulation, and sol-gel reactions can also be used for microplastic removal. Biological methods such as the use of microorganisms and fungi are also effective for microplastic degradation. Advanced filtration technologies like a combination of membrane bioreactor and activated sludge method show high microplastic removal efficiency. 

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