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A thorough investigation into the biophysical properties of four animal-derived clinical surfactant preparations was conducted through constrained drop surfactometry under physiologically relevant conditions. This comparative study unveiled unique in vitro biophysical characteristics among these clinical surfactants, establishing correlations between their chemical composition, lateral film structure, and biophysical functionality. The acquired knowledge offers essential insights for the precise and personalized design of clinical surfactant for the treatment of respiratory distress syndrome and other respiratory conditions.
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Constrained drop surfactometry for studying adsorbed pulmonary surfactant at physiologically relevant high concentrations
An in vitro biophysical model, called constrained drop surfactometry, was developed to study the biophysical properties, ultrastructure, and topography of the pulmonary surfactant film adsorbed from the subphase at physiologically relevant high surfactant concentrations of 10–35 mg/mL. These results suggest that the biophysical function of multilayers formed after de novo adsorption is to act as a buffer zone to store surface-active materials ejected from the interfacial monolayer under extreme conditions such as deep breathing.
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- Award ID(s):
- 2011317
- PAR ID:
- 10535856
- Publisher / Repository:
- American Physiology Society
- Date Published:
- Journal Name:
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Volume:
- 325
- Issue:
- 4
- ISSN:
- 1040-0605
- Page Range / eLocation ID:
- L508 to L517
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1152/ajplung.00101.2023
