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The multi-institutional Fly-CURE project is an undergraduate genetics research initiative centered on Drosophila melanogaster as a model organism. This study aimed to characterize and map mutations discovered through a Flp/FRT EMS screen to investigate complex interactions among genes associated with cell division, growth, and apoptosis leading to abnormal cell proliferation. The F.1.1 mosaic phenotype resulted in a rough eye phenotype with an overall decrease in representation of mutant tissue. To genetically map the location of the F.1.1 mutation, flies with genotype FRT42D,F.1.1,Dark82/CyO were crossed with the Bloomington 2R Deficiency Kit. The resultant F1 progeny were analyzed to pinpoint mapping deficiencies. The genomic region containing the Patronin gene was identified and sequencing confirmed the novel allele of PatroninF.1.1.
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Genetic Mapping of prodE.3.3, a New Lethal Allele of prod
The E.3.3 mutation was generated in a Flp/FRT EMS screen for conditional mutations that cause growth and developmental defects in a genetic background that blocks apoptosis. The mutations were conditional, based on the Dark82allele being present on the starting chromosome, and blocking canonical apoptosis in a homozygous state. The E.3.3 mosaic eyes exhibit defects in eye development including patches of rough eye and irregular surface structure. Whole Genome Sequencing and complementation mapping revealed E.3.3 as an allele of prod. Prod is a DNA-binding protein that binds satellite repeats and is involved in chromocenter formation during mitosis. Here we present a novel allele of prod, prodE.3.3, that disrupts the functional region of the Prod protein resulting in disruption of typical eye structure, likely due to disruption of chromatid separation during development.
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- Award ID(s):
- 2021146
- PAR ID:
- 10543608
- Author(s) / Creator(s):
- ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; more »
- Publisher / Repository:
- microPublication Biology
- Date Published:
- Journal Name:
- microPublication biology
- ISSN:
- 2578-9430
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.17912/micropub.biology.001236
