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Title: A conserved transcription factor controls gluconeogenesis via distinct targets in hypersaline-adapted archaea with diverse metabolic capabilities

Timely regulation of carbon metabolic pathways is essential for cellular processes and to prevent futile cycling of intracellular metabolites. InHalobacterium salinarum, a hypersaline adapted archaeon, a sugar-sensing TrmB family protein controls gluconeogenesis and other biosynthetic pathways. Notably,Hbt. salinarumdoes not utilize carbohydrates for energy, uncommon among Haloarchaea. We characterized a TrmB-family transcriptional regulator in a saccharolytic generalist,Haloarcula hispanica, to investigate whether the targets and function of TrmB, or its regulon, is conserved in related species with distinct metabolic capabilities. InHar. hispanica, TrmB binds to 15 sites in the genome and induces the expression of genes primarily involved in gluconeogenesis and tryptophan biosynthesis. An important regulatory control point inHbt. salinarum, activation ofppsAand repression ofpykA, is absent inHar. hispanica. Contrary to its role inHbt. salinarumand saccharolytic hyperthermophiles, TrmB does not act as a global regulator: it does not directly repress the expression of glycolytic enzymes, peripheral pathways such as cofactor biosynthesis, or catabolism of other carbon sources inHar. hispanica. Cumulatively, these findings suggest rewiring of the TrmB regulon alongside metabolic network evolution in Haloarchaea.

 
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Award ID(s):
1651117 1936024
PAR ID:
10543694
Author(s) / Creator(s):
; ; ;
Editor(s):
Albers, Sonja
Publisher / Repository:
Public Library of Science
Date Published:
Journal Name:
PLOS Genetics
Volume:
20
Issue:
1
ISSN:
1553-7404
Page Range / eLocation ID:
e1011115
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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