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Title: Skeletal muscle-on-a-chip in microgravity as a platform for regeneration modeling and drug screening
Microgravity has been shown to lead to both muscle atrophy and impaired muscle regeneration. The purpose was to study the efficacy of microgravity to model impaired muscle regeneration in an engineered muscle platform and then to demonstrate the feasibility of performing drug screening in this model. Engineered human muscle was launched to the International Space Station National Laboratory, where the effect of microgravity exposure for 7 days was examined by transcriptomics and proteomics approaches. Gene set enrichment analysis of engineered muscle cultured in microgravity, compared to normal gravity conditions, highlighted a metabolic shift toward lipid and fatty acid metabolism, along with increased apoptotic gene expression. The addition of pro-regenerative drugs, insulin-like growth factor-1 (IGF-1) and a 15-hydroxyprostaglandin dehydrogenase inhibitor (15-PGDH-i), partially inhibited the effects of microgravity. In summary, microgravity mimics aspects of impaired myogenesis, and the addition of these drugs could partially inhibit the effects induced by microgravity.  more » « less
Award ID(s):
1829534 2227614
PAR ID:
10545100
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
Lippincott Williams & Wilkins
Date Published:
Journal Name:
Stem Cell Reports
Volume:
19
Issue:
8
ISSN:
2213-6711
Page Range / eLocation ID:
1061 to 1073
Subject(s) / Keyword(s):
engineered muscle, sarcopenia, atrophy, muscle, regeneration, microgravity, space, drug screening
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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