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This content will become publicly available on September 18, 2025

Title: Effects of periodic bottlenecks on the dynamics of adaptive evolution in microbial populations

Population bottlenecks can impact the rate of adaptation in evolving populations. On the one hand, each bottleneck reduces the genetic variation that fuels adaptation. On the other hand, each founder that survives a bottleneck can undergo more generations and leave more descendants in a resource-limited environment, which allows surviving beneficial mutations to spread more quickly. A theoretical model predicted that the rate of fitness gains should be maximized using ~8-fold dilutions. Here we investigate the impact of repeated bottlenecks on the dynamics of adaptation using numerical simulations and experimental populations ofEscherichia coli. Our simulations confirm the model’s prediction when populations evolve in a regime where beneficial mutations are rare and waiting times between successful mutations are long. However, more extreme dilutions maximize fitness gains in simulations when beneficial mutations are common and clonal interference prevents most of them from fixing. To examine these predictions, we propagated 48E. colipopulations with 2-, 8-, 100-, and 1000-fold dilutions for 150 days. Adaptation began earlier and fitness gains were greater with 100- and 1000-fold dilutions than with 8-fold dilutions, consistent with the simulations when beneficial mutations are common. However, the selection pressures in the 2-fold treatment were qualitatively different from the other treatments, violating a critical assumption of the model and simulations. Thus, varying the dilution factor during periodic bottlenecks can have multiple effects on the dynamics of adaptation caused by differential losses of diversity, different numbers of generations, and altered selection.

 
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Award ID(s):
1813069
PAR ID:
10546200
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
Microbiology
Date Published:
Journal Name:
Microbiology
Volume:
170
Issue:
9
ISSN:
1350-0872
Page Range / eLocation ID:
001494
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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