More Like this

1. Complete Genome Sequence of Rhodococcus qingshengii Strain CL-05, Isolated from Concrete

   https://doi.org/10.1128/MRA.00376-21  

   Kiledal EA, McDermott SG (May 2021, Microbiology resource announcements)

   null (Ed.)

   Here, we report the complete genome sequence of Rhodococcus qingshengii strain CL-05, which was isolated from pavement concrete in Newark, Delaware. The genome consists of a 6.29-Mbp chromosome and one plasmid (123,183 bp), encodes a total of 5,859 predicted proteins, and has a GC content of 62.5%. 

   more » « less
2. Mechanistic Insights into DyPB from Rhodococcus jostii RHA1 Via Kinetic Characterization

   https://doi.org/10.1021/acscatal.1c00703  

   Shrestha, Ruben; Jia, Kaimin; Khadka, Samiksha; Eltis, Lindsay D.; Li, Ping (May 2021, ACS Catalysis)

   null (Ed.)
3. Complete Genome Sequence of Rhodococcus opacus Strain MoAcy1 (DSM 44186), an Aerobic Acetylenotroph Isolated from Soil

   https://doi.org/10.1128/MRA.00814-21  

   Sutton, John M; Bushman, Timothy J; Akob, Denise M; Fierst, Janna L (January 2022, Microbiology Resource Announcements)

   Pritchard, Leighton (Ed.)

   We report the genome ofRhodococcus opacusstrain MoAcy1 (DSM 44186), an aerobic soil isolate capable of using acetylene as its primary carbon and energy source (acetylenotrophy). The genome is composed of a single circular chromosome of ∼8 Mbp and two closed plasmids, with a G+C content of 67.3%. 

   more » « less
4. The Fe-type nitrile hydratase from Rhodococcus equi TG328-2 forms an alpha-activator protein complex

   https://doi.org/10.1007/s00775-020-01806-y  

   Lankathilaka, K. P.; Bennett, Brian; Holz, Richard C. (September 2020, JBIC Journal of Biological Inorganic Chemistry)

   null (Ed.)
5. Desulfonation and defluorination of 6:2 fluorotelomer sulfonic acid (6:2 FTSA) by Rhodococcus jostii RHA1: Carbon and sulfur sources, enzymes, and pathways

   https://doi.org/10.1016/j.jhazmat.2021.127052  

   Shih-Hung Yang Ying Shi Mark Strynar Kung-Hui Chu (February 2022, Journal of hazardous materials)

   6:2 fluorotelomer sulfonic acid (6:2 FTSA) is one per- and poly-fluoroalkyl substances commonly detected in the environment. While biotransformation of 6:2 FTSA has been reported, factors affecting desulfonation and defluorination of 6:2 FTSA remain poorly understood. This study elucidated the effects of carbon and sulfur sources on the gene expression of Rhodococcus jostii RHA1 which is responsible for the 6:2 FTSA biotransformation. While alkane monooxygenase and cytochrome P450 were highly expressed in ethanol-, 1-butanol-, and n-octane-grown RHA1 in sulfur-rich medium, these cultures only defluorinated 6:2 fluorotelomer alcohol but not 6:2 FTSA, suggesting that the sulfonate group in 6:2 FTSA hinders enzymatic defluorination. In sulfur-free growth media, alkanesulfonate monooxygenase was linked to desulfonation of 6:2 FTSA; while alkane monooxygenase, haloacid dehalogenase, and cytochrome P450 were linked to defluorination of 6:2 FTSA. The desulfonation and defluorination ability of these enzymes toward 6:2 FTSA were validated through heterologous gene expression and in vitro assays. Four degradation metabolites were confirmed and one was identified as a tentative metabolite. The results provide a new understanding of 6:2 FTSA biotransformation by RHA1. The genes encoding these desulfonating- and defluorinating-enzymes are potential markers to be used to assess 6:2 FTSA biotransformation in the environment. 

   more » « less