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1. Closed circular genome sequence of a Microcystis aeruginosa PCC7806 Δ mcyB (UTK) non-toxic mutant

   https://doi.org/10.1128/MRA.00700-23  

   Stark, Gwendolyn F; Truchon, Alexander R; Dittmann, Elke; Wilhelm, Steven W (November 2023, Microbiology Resource Announcements)

   Becket, Elinne (Ed.)

   ABSTRACT Here we report the complete, closed genome of the non-toxicMicrocystis aeruginosaPCC7806 ΔmcyBmutant strain. This genome is 5,103,923 bp long, with a GC content of 42.07%. Compared to the published wild-type genome (Microcystis aeruginosaPCC7806SL), there is evidence of accumulated mutations beyond the inserted chloramphenicol resistance marker. 

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2. Aureococcus anophagefferens strain CCMP1851: draft genome of a second Kratosvirus quantuckense- susceptible host strain for an emerging host–giant virus model system

   https://doi.org/10.1128/mra.00292-24  

   Chase, Emily E; Truchon, Alexander R; Schepens, William W; Wilhelm, Steven W (June 2024, Microbiology Resource Announcements)

   Hudson, André O (Ed.)

   ABSTRACT Here, we report the draft genome ofAureococcus anophagefferensstrain CCMP1851, which is susceptible to the virusKratosvirus quantuckense. CCMP1851 complements an available genome for a virus-resistant strain (CCMP1850) isolated from the same bloom. Future studies can now use this genome to examine genetic hints of virus resistance and susceptibility. 

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3. Oogenesis and germinal bed morphology of the brown anole ( A . sagrei )

   https://doi.org/10.1002/dvdy.70112  

   Kircher, Bonnie K; Weberling, Antonia; Vance, Erin J; Shylo, Natalia A; Starr, Katherine; Griffin, Zoe B; Wilson, Hannah; McClain, Melainia; Hollfelder, Florian; Williams, Suzannah A; et al (January 2026, Developmental Dynamics)

   Abstract BackgroundThe brown anole is a model species of the genusAnolis, a squamate (encompassing lizards and snakes) group widely studied in evolutionary, behavioral, and developmental biology. Full genome annotation, the establishment of gene editing techniques, and comprehensive description of reproductive tract morphology and embryogenesis in this species have laid the foundation for functional studies. However, analysis of brown anole oogenesis is still required and vital to optimize genome modification, mutant line establishment, and analyses of the evolution of reproductive developmental mechanisms. ResultsHere, we characterize ovary morphology and gametogenesis in the female brown anole,Anolis sagrei,using brightfield imaging, microCT, histology staining, electron microscopy, and confocal imaging. We define 10 stages of oocyte maturation, which commences inside the oogonial nest within the germinal bed and concludes with the mature follicle ready to ovulate based on follicle size, yolk acquisition, and follicular, cellular, and basement membrane architecture. ConclusionsWe describe the complete oogenesis of the brown anole in 10 stages and report that oogenesis is highly conserved within iguanians, a suborder of lizards. With our staging framework, we lay the foundation for functional studies of oogenesis and optimized gene‐editing. 

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4. Complete genome sequence of Bacillus thuringiensis strain RC340, isolated from a temperate forest soil sample in New England

   https://doi.org/10.1128/MRA.00607-23  

   Sullivan, Brendan; Kitzmiller, Claire E.; Tran, Wyatt C.; Choudoir, Mallory; Simoes, Rachel; Dayarathne, Nipuni; DeAngelis, Kristen M. (November 2023, Microbiology Resource Announcements)

   Rasko, David (Ed.)

   ABSTRACT The complete genome sequence ofBacillus thuringiensisstrain RC340, isolated from an environmental microbiology experiment soil sample is presented here.B. thuringiensisstrain RC340 sequenced by GridION consists of a single genome consisting of 5.86 million bases, 8,152 predicted genes, and 0.23% contamination. 

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5. Chromosome‐scale reference genome of Pectocarya recurvata , the species with the smallest reported genome size in Boraginaceae

   https://doi.org/10.1002/aps3.70008  

   Northing, Poppy C; Pelosi, Jessie A; Venable, D Lawrence; Dlugosch, Katrina M (May 2025, Applications in Plant Sciences)

   Abstract PremisePectocarya recurvata(Boraginaceae, subfamily Cynoglossoideae), a species native to the Sonoran Desert (North America), has served as a model system for a suite of ecological and evolutionary studies. However, no reference genomes are currently available in Cynoglossoideae. A high‐quality reference genome forP. recurvatawould be valuable for addressing questions in this system and across broader taxonomic scales. MethodsUsing PacBio HiFi sequencing, we assembled a reference genome forP. recurvataand annotated coding regions with full‐length transcripts from an Iso‐Seq library. We assessed genome completeness with BUSCO andk‐mer analysis, and estimated the genome size of six individuals using flow cytometry. ResultsThe chromosome‐scale genome assembly forP. recurvatawas 216.0 Mbp long (N50 = 12.1 Mbp). Previous observations indicatedP. recurvatais 2n = 24. Our assembly included 12 primary contigs (158.3 Mbp) containing 30,655 genes with telomeres at 23 out of 24 ends. Flow cytometry measurements from the same population included two plants with 1C = 196.9 Mbp, the smallest measured for Boraginaceae, and four with 1C = 385.8 Mbp, which is consistent with tetraploidy in this population. DiscussionTheP. recurvatagenome assembly and annotation provide a high‐quality genomic resource in a sparsely represented area of the angiosperm phylogeny. This new reference genome will facilitate answering open questions in ecophysiology, biogeography, and systematics. 

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