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Title: Biochemical analysis of the TPS-a subfamily in Medicago truncatula
Terpenes are important mediators of plant chemical response to environmental cues. Here, we describe the genome-wide identification and biochemical characterization of TPS-a members inMedicago truncatula, a model legume crop. Genome mining identified thirty-nine full-length terpene synthases with a significant number predicted to produce monoterpenes and sesquiterpenes. Biochemical characterization of the TPS-a subfamily associated with sesquiterpene biosynthesis revealed such compounds, that exhibit substantial biological activity in other plants. Gene expression analysis using qPCR and theMedicagogene atlas illustrated distinct tissue and time-based variation in expression in leaves and roots. Together our work establishes the gene-to-metabolite relationships for sesquiterpene synthases inM. truncatula. Understanding the biosynthetic capacity is a foundational step to defining the ecological roles of this important family of compounds.  more » « less
Award ID(s):
2300060
PAR ID:
10571764
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
Frontiers in Plant Science
Date Published:
Journal Name:
Frontiers in Plant Science
Volume:
15
ISSN:
1664-462X
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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