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Title: Population epigenetics: DNA methylation in the plant omics era
Abstract DNA methylation plays an important role in many biological processes. The mechanisms underlying the establishment and maintenance of DNA methylation are well understood thanks to decades of research using DNA methylation mutants, primarily in Arabidopsis (Arabidopsis thaliana) accession Col-0. Recent genome-wide association studies (GWASs) using the methylomes of natural accessions have uncovered a complex and distinct genetic basis of variation in DNA methylation at the population level. Sequencing following bisulfite treatment has served as an excellent method for quantifying DNA methylation. Unlike studies focusing on specific accessions with reference genomes, population-scale methylome research often requires an additional round of sequencing beyond obtaining genome assemblies or genetic variations from whole-genome sequencing data, which can be cost prohibitive. Here, we provide an overview of recently developed bisulfite-free methods for quantifying methylation and cost-effective approaches for the simultaneous detection of genetic and epigenetic information. We also discuss the plasticity of DNA methylation in a specific Arabidopsis accession, the contribution of DNA methylation to plant adaptation, and the genetic determinants of variation in DNA methylation in natural populations. The recently developed technology and knowledge will greatly benefit future studies in population epigenomes.  more » « less
Award ID(s):
2228767
PAR ID:
10573028
Author(s) / Creator(s):
;
Publisher / Repository:
Oxford Academic
Date Published:
Journal Name:
Plant Physiology
Volume:
194
Issue:
4
ISSN:
0032-0889
Page Range / eLocation ID:
2039 to 2048
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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