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Abstract The force-generating capacity of muscle depends upon many factors including the actin-myosin filament overlap due to the relative length of the sarcomere. Consequently, the force output of a muscle may vary throughout its range of motion, and the body posture allowing maximum force generation may differ even in otherwise similar species. We hypothesized that corn snakes would show an ontogenetic shift in sarcomere length range from being centered on the plateau of the length-tension curve in small individuals to being on the descending limb in adults. Sarcomere lengths across the plateau would be advantageous for locomotion, while the descending limb would be advantageous for constriction due to the increase in force as the coil tightens around the prey. To test this hypothesis, we collected sarcomere lengths from freshly euthanized corn snakes, preserving segments in straight and maximally curved postures, and quantifying sarcomere length via light microscopy. We dissected 7 muscles (spinalis, semispinalis, multifidus, longissimus dorsi, iliocostalis (dorsal and ventral), and levator costae) in an ontogenetic series of corn snakes (mass = 80–335 g) at multiple regions along the body (anterior, middle, and posterior). Our data shows all of the muscles analyzed are on the descending limb of the length-tension curve at rest across all masses, regions, and muscles analyzed, with muscles shortening onto or past the plateau when flexed. While these results are consistent with being advantageous for constriction at all sizes, there could also be unknown benefits of this sarcomere arrangement for locomotion or striking.
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This content will become publicly available on March 1, 2026
Fluoromicrometry reveals minimal influence of tendon elasticity during snake locomotion
ABSTRACT Multiarticular muscle systems are widespread across vertebrates, including in their necks, digits, tails and trunks. In secondarily limbless tetrapods, the multiarticular trunk muscles power nearly all behaviors. Using snakes as a study system, we previously used anatomical measurements and mathematical modeling to derive an equation relating multiarticular trunk muscle shortening to postural change. However, some snake trunk muscles have long, thin tendinous connections, raising the possibility of elastic energy storage, which could lead to a decoupling of muscle length change from joint angle change. The next step, therefore, is to determine whether in vivo muscle shortening produces the postural changes predicted by mathematical modeling. A departure from predictions would implicate elastic energy storage. To test the relationship between muscle strain and posture in vivo, we implanted radio-opaque metal beads in three muscles of interest in four corn snakes (Pantherophis guttatus), then recorded X-ray videos to directly measure muscle shortening and vertebral column curvature during locomotion. Our in vivo results produced evidence that elastic energy storage does not play a substantial role in corn snake lateral undulation or tunnel concertina locomotion. The ability to predict muscle shortening directly from observed posture will facilitate future work. Moreover, the generality of our equation, which uses anatomical values that can be measured in many types of animals, means that our framework for understanding multiarticular muscle function can be applied in numerous study systems to provide a stronger mechanistic understanding of organismal function.
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- PAR ID:
- 10574819
- Publisher / Repository:
- The Company of Biologists
- Date Published:
- Journal Name:
- Journal of Experimental Biology
- Volume:
- 228
- Issue:
- 5
- ISSN:
- 0022-0949
- Subject(s) / Keyword(s):
- Snake biomechanics fluoromicrometry muscle
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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