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Sleep is ancient and genetically conserved across phylogeny. Neuropeptide signaling plays a fundamental role in the regulation of sleep for mammals, fish, and invertebrates like Caenorhabditis elegans. Developmentally timed-sleep and stress-induced sleep of C. elegans are controlled by distinct and overlapping neuropeptide pathways. The RPamide neuropeptides nlp-2, nlp-22, and nlp-23, play antagonistic roles during the regulation of developmentally-timed sleep, however, their role in stress-induced sleep has not been explored. These genes are linked on the X chromosome, which has made genetic analyses challenging. Here we used CRISPR to generate new alleles of nlp-22 and nlp-23, nlp-22;nlp-23 double mutants, and nlp-2;nlp-22;nlp-23 triple mutants. Confirming previous studies, we find that nlp-22 is required for developmentally-timed sleep, and show that nlp-23 is also required. However, all three genes are dispensable for stress-induced sleep.
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Data and code: Herbivory exacerbates pollen limitation by isolating unconsumed plants from prospective mates
Data and R code necessary to replicate figures and analyses presented in the following manuscript: Beck and Wagenius. In press. Herbivory exacerbates pollen limitation by isolating unconsumed plants from prospective mates. American Journal of Botany.
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- PAR ID:
- 10575055
- Publisher / Repository:
- figshare
- Date Published:
- Subject(s) / Keyword(s):
- Population ecology
- Format(s):
- Medium: X Size: 139856 Bytes
- Size(s):
- 139856 Bytes
- Right(s):
- Creative Commons Attribution 4.0 International
- Sponsoring Org:
- National Science Foundation
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