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PremiseThe ability to sequence genome‐scale data from herbarium specimens would allow for the economical development of data sets with broad taxonomic and geographic sampling that would otherwise not be possible. Here, we evaluate the utility of a basic double‐digest restriction site–associatedDNAsequencing (ddRADseq) protocol usingDNAs from four genera extracted from both silica‐dried and herbarium tissue. MethodsDNAs fromDraba,Boechera,Solidago, andIlexwere processed with a ddRADseq protocol. The effects ofDNAdegradation, taxon, and specimen age were assessed. ResultsAlthough taxon, preservation method, and specimen age affected data recovery, large phylogenetically informative data sets were obtained from the majority of samples. DiscussionThese results suggest that herbarium samples can be incorporated into ddRADseq project designs, and that specimen age can be used as a rapid on‐site guide for sample choice. The detailed protocol we provide will allow users to pursue herbarium‐based ddRADseq projects that minimize the expenses associated with fieldwork and sample evaluation.
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This content will become publicly available on March 26, 2026
Dental Wear and Molar Pulp Volume Reduction in Macaca fascicularis
ABSTRACT ObjectivesOdontoblasts lining the pulp cavity deposit dentine throughout life, meaning the volume of the pulp cavity decreases with age. Primates with more abrasive diets have relatively higher molar pulp volume in their unworn molars than those with less abrasive diets. We propose that species with more abrasive diets deposit additional dentine across their lifespans to help resist wear and extend the effective lifespan of their molars. Whereas both age and wear affect dentine deposition, it is unknown which of these two variables has the greater impact. Materials and MethodsWe measured pulp volume and calculated wear from micro‐CT scans of lower first molars of captive‐bredMacaca fascicularis(n = 13) of known age. We used reduced major axis regressions (alpha = 0.05) to test if age or wear was a better predictor of pulp volume. ResultsBoth variables have a significant negative relationship with pulp volume (age:p = 0.004,R2 = 0.546; wear:p < 0.001,R2 = 0.890). A mixed linear model of pulp volume against wear as main effect and age as covariant had a non‐significant interaction effect (p = 0.078) and confirmed that both age (p = 0.030) and wear (p = 0.004) are significantly negatively correlated with pulp volume. DiscussionResults suggest that whereas pulp volume decreases with age, wear is more strongly correlated with decreasing pulp volume. These findings have implications for interpreting odontoblast activity in response to sensory feedback and the relationship between pulp volume and diet. These results also have implications for using molar pulp volume to estimate age at death in humans.
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- PAR ID:
- 10579662
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- American Journal of Biological Anthropology
- Volume:
- 186
- Issue:
- 3
- ISSN:
- 2692-7691
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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