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This content will become publicly available on December 20, 2025

Title: The three-dimensional genome drives the evolution of asymmetric gene duplicates via enhancer capture-divergence
Previous evolutionary models of duplicate gene evolution have overlooked the pivotal role of genome architecture. Here, we show that proximity-based regulatory recruitment by distally duplicated genes is an efficient mechanism for modulating tissue-specific production of preexisting proteins. By leveraging genomic asymmetries, we performed a coexpression analysis onDrosophila melanogastertissue data to show the generality of enhancer capture-divergence (ECD) as a significant evolutionary driver of asymmetric, distally duplicated genes. We use the recently evolved geneHP6/Umbreaas an example of the ECD process. By assaying genome-wide chromosomal conformations in multipleDrosophilaspecies, we show thatHP6/Umbreawas inserted near a preexisting, long-distance three-dimensional genomic interaction. We then use this data to identify a newly found enhancer (FLEE1), buried within the coding region of the highly conserved, essential geneMFS18, that likely neofunctionalizedHP6/Umbrea. Last, we demonstrate ancestral transcriptional coregulation ofHP6/Umbrea’s future insertion site, illustrating how enhancer capture provides a highly evolvable, one-step solution to Ohno’s dilemma.  more » « less
Award ID(s):
2410289
PAR ID:
10580276
Author(s) / Creator(s):
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publisher / Repository:
American Association for the Advancement of Science
Date Published:
Journal Name:
Science Advances
Volume:
10
Issue:
51
ISSN:
2375-2548
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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