This revision is based on sampling efforts over the past three decades in the southern Appalachian Mountains which have providedNesticus(Araneae, Nesticidae) collections of approximately 2100 adult specimens from more than 475 unique collecting events. Using a “morphology first” framework we examined recently collected specimens plus museum material to formulate morphology-based species hypotheses for putative new taxa (discovery phase). Using sequence capture of nuclear ultraconserved elements (UCEs) we analyzed 801 nuclear loci to validate new (and prior) morphology-based species hypotheses (validation phase) and reconstructed a robust backbone phylogeny including all described and new species. Sanger sequencing and UCE-bycatch were also used to gather mitochondrial data for more than 240 specimens. Based on our integrative taxonomic framework ten newNesticusspecies are herein described, includingN. binfordaesp. nov.,N. bondisp. nov.,N. caneisp. nov.,N. cherokeensissp. nov.,N. dellingerisp. nov.,N. dykemanaesp. nov.,N. jemisinaesp. nov.,N. lowderisp. nov.,N. roanensissp. nov., andN. templetonisp. nov.Previously unknown males are also described forN. bishopiGertsch, 1984,N. crosbyiGertsch, 1984, andN. silvanusGertsch, 1984, as well as the previously unknown female forN. mimusGertsch, 1984. Based on combined evidenceN. cooperiGertsch, 1984 is placed in synonymy withN. reclususGertsch, 1984. Overall, the montane radiation of AppalachianNesticusreveals a general lack of species sympatry and compelling biogeographic patterns. Several regionalNesticustaxa are rare, microendemic habitat specialists that deserve conservation attention and detailed future monitoring as conservation sentinels. 
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                            Remarks on the diversity of Hysteromorpha Lutz 1931 (Digenea, Diplostomidae), with erection of a new species from South America
                        
                    
    
            Abstract HysteromorphaLutz, 1931 is a small but broadly distributed genus of diplostomoidean digeneans parasitic as adults primarily in cormorants, but also reported from some other fish-eating birds. Their metacercariae were found in a variety of freshwater fishes as second intermediate hosts. Prior to this study, the genus included only 3 nominal species, 2 of them distributed in the Old World and 1 in the New World. We obtained sequences of partial mitochondrialcox1 gene and nuclear rDNA operon from new specimens collected in Europe, North and South America and used them for species comparisons and phylogenetic analysis. We also examined morphology of our newly collected specimens as well as museum specimens. Our analysis has demonstrated that at least 2 (likely 3) species ofHysteromorphaare distributed in the Americas.Hysteromorphasp. previously sequenced from larval stages, clearly represents a new species named hereinHysteromorpha ostrowskiaen. sp.Achatz, Locke et Tkach. Morphology of adults of the new species was earlier described in sufficient detail under the nameHysteromorpha triloba(Rudolphi, 1819). Our analyses also suggest the presence of another unknownHysteromorphasp. in North America represented bycox1 sequence from southeastern Canada (GenBank JF769473), but no morphological vouchers are available for this species. 
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                            - Award ID(s):
- 1845021
- PAR ID:
- 10581163
- Publisher / Repository:
- Springer Science + Business Media
- Date Published:
- Journal Name:
- Systematic Parasitology
- Volume:
- 102
- Issue:
- 2
- ISSN:
- 0165-5752
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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