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Title: From molecular descriptions to cellular functions of intrinsically disordered protein regions
Molecular descriptions of intrinsically disordered protein regions (IDRs) are fundamental to understanding their cellular functions and regulation. NMR spectroscopy has been a leading tool in characterizing IDRs at the atomic level. In this review, we highlight recent conceptual breakthroughs in the study of IDRs facilitated by NMR and discuss emerging NMR techniques that bridge molecular descriptions to cellular functions. First, we review the assemblies formed by IDRs at various scales, from one-to-one complexes to non-stoichiometric clusters and condensates, discussing how NMR characterizes their structural dynamics and molecular interactions. Next, we explore several unique interaction modes of IDRs that enable regulatory mechanisms such as selective transport and switch-like inhibition. Finally, we highlight recent progress in solid-state NMR and in-cell NMR on IDRs, discussing how these methods allow for atomic characterization of full-length IDR complexes in various phases and cellular environments. This review emphasizes recent conceptual and methodological advancements in IDR studies by NMR and offers future perspectives on bridging the gap between in vitro molecular descriptions and the cellular functions of IDRs.  more » « less
Award ID(s):
1932730
PAR ID:
10582905
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
American Institute of Physics
Date Published:
Journal Name:
Biophysics Reviews
Volume:
5
Issue:
4
ISSN:
2688-4089
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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