skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Explore Research Products in the PAR
It may take a few hours for recently added research products to appear in PAR search results.


Title: Silver-Platinum Hollow Nanoparticles as Labels for Colorimetric Lateral Flow Assay
ArticleSilver-Platinum Hollow Nanoparticles as Labels for Colorimetric Lateral Flow AssayJinfeng Zhou 1, Shikuan Shao 1, Zhiyuan Wei 1 and Xiaohu Xia 1,2,*1 Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA2 NanoScience Technology Center, University of Central Florida, Orlando, FL 32816, USA* Correspondence: xiaohu.xia@ucf.eduReceived: 23 September 2024; Revised: 7 November 2024; Accepted: 12 November 2024; Published: 18 November 2024 Abstract: Colorimetric lateral flow assay (CLFA) has been a widely recognized point-of-care testing technology over the past few decades. Driven by the increasing demand in various biomedical applications, it is urgently needed to develop CLFAs with high sensitivities and low costs. In this work, we report a type of CLFA that relies on unique colorimetric labels—silver-platinum hollow nanoparticles (Ag-Pt HNPs). The Ag-Pt HNPs possess intrinsic enzyme-like catalytic activities, providing the Ag-Pt HNP-based CLFA with strong color signal and thus a high sensitivity. Meanwhile, the Ag-Pt HNPs have hollow interiors and are mainly composed of less expensive silver, making the Ag-Pt HNP-based CLFA cost-effective. Using prostate-specific antigen (PSA) as a model disease biomarker, the Ag-Pt HNP-based CLFA achieved a high sensitivity with a detection limit at the low picogram-per-milliliter level. Potential application of the CLFA in clinical diagnosis was demonstrated by detecting PSA from human serum samples.  more » « less
Award ID(s):
2234873
PAR ID:
10593310
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
Scilight Press
Date Published:
Journal Name:
Materials and Interfaces
ISSN:
2982-2394
Page Range / eLocation ID:
2
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. ; ; ; ; ; ; (, American Society for Engineering Education Annual Conference: NSF Poster Session)
    The Florida IT Graduation Attainment Pathways (Flit-GAP), an NSF S-STEM, Track 3 grant effort, involves three public metropolitan institutions from Florida’s three most populous areas: Florida International University (FIU) in Miami, University of Central Florida (UCF) in Orlando, and University of South Florida (USF) in Tampa. Flit-GAP supports up to 50 students per year for each of the first 3 years of the project’; recruits are juniors from Computer Science, Information Technology, Computer Engineering, and Cybersecurity, and other computing majors. The relationship among the three institutions is formalized as the Consortium of Florida Metropolitan Research Universities. The consortium is a strategic priority of each institution. In Year 1, 42 students participated in the scholarship program at the three institutions (16 FIU; 14 UCF; 11 USF). 
    more » « less
  2. ; ; ; ; ; ; (, American Society for Engineering Education Annual Conference: NSF Poster Session)
    The Florida IT Graduation Attainment Pathways (Flit-GAP), an NSF S-STEM, Track 3 grant effort, involves three public metropolitan institutions from Florida’s three most populous areas: Florida International University (FIU) in Miami, University of Central Florida (UCF) in Orlando, and University of South Florida(USF) in Tampa. Flit-GAP supports up to 50 students per year for each of the first 3 years of the project’; recruits are juniors from Computer Science, Information Technology, Computer Engineering, and Cybersecurity, and other computing majors. The relationship among the three institutions is formalized as the Consortium of Florida Metropolitan Research Universities. The consortium is a strategic priority of each institution. In Year 1, 42 students participated in the scholarship program at the three institutions (16 FIU; 14 UCF; 11 USF). 
    more » « less
  3. ; ; ; ; ; ; (, American Society for Engineering Education Annual Conference: NSF Poster Session)
    The Florida IT Graduation Attainment Pathways (Flit-GAP), an NSF S-STEM, Track 3 grant effort, involves three public metropolitan institutions from Florida’s three most populous areas: Florida International University (FIU) in Miami, University of Central Florida (UCF) in Orlando, and University of South Florida (USF) in Tampa. Flit-GAP supports up to 50 students per year for each of the first 3 years of the project’; recruits are juniors from Computer Science, Information Technology, Computer Engineering, and Cybersecurity, and other computing majors. The relationship among the three institutions is formalized as the Consortium of Florida Metropolitan Research Universities. The consortium is a strategic priority of each institution. In Year 1, 42 students participated in the scholarship program at the three institutions (16 FIU; 14 UCF; 11 USF). 
    more » « less
  4. ; ; ; (, Chemosensors)
    Colorimetric enzyme-linked immunosorbent assay (ELISA) has been widely applied as the gold-standard method for cytokine detection for decades. However, it has become a critical challenge to further improve the detection sensitivity of ELISA, as it is limited by the catalytic activity of enzymes. Herein, we report an enhanced colorimetric ELISA for ultrasensitive detection of interleukin-6 (IL-6, as a model cytokine for demonstration) using Pd@Pt core@shell nanodendrites (Pd@Pt NDs) as peroxidase nanomimics (named “Pd@Pt ND ELISA”), pushing the sensitivity up to femtomolar level. Specifically, the Pd@Pt NDs are rationally engineered by depositing Pt atoms on Pd nanocubes (NCs) to generate rough dendrite-like Pt skins on the Pd surfaces via Volmer–Weber growth mode. They can be produced on a large scale with highly uniform size, shape, composition, and structure. They exhibit significantly enhanced peroxidase-like catalytic activity with catalytic constants (Kcat) more than 2000-fold higher than those of horseradish peroxidase (HRP, an enzyme commonly used in ELISA). Using Pd@Pt NDs as the signal labels, the Pd@Pt ND ELISA presents strong colorimetric signals for the quantitative determination of IL-6 with a wide dynamic range of 0.05–100 pg mL−1 and an ultralow detection limit of 0.044 pg mL−1 (1.7 fM). This detection limit is 21-fold lower than that of conventional HRP-based ELISA. The reproducibility and specificity of the Pd@Pt ND ELISA are excellent. More significantly, the Pd@Pt ND ELISA was validated for analyzing IL-6 in human serum samples with high accuracy and reliability through recovery tests. Our results demonstrate that the colorimetric Pd@Pt ND ELISA is a promising biosensing tool for ultrasensitive determination of cytokines and thus is expected to be applied in a variety of clinical diagnoses and fundamental biomedical studies. 
    more » « less
  5. ; (, Scientific Reports)
    Abstract In this work, a low power microcontroller-based near field communication (NFC) interfaced with a flexible abiotic glucose hybrid fuel cell is designed to function as a battery-less glucose sensor. The abiotic glucose fuel cell is fabricated by depositing colloidal platinum (co–Pt) on the anodic region and silver oxide nanoparticles-multiwalled carbon nanotubes (Ag 2 O-MWCNTs) composite on the cathodic region. The electrochemical behavior is characterized using cyclic voltammetry and chronoamperometry. This glucose hybrid fuel cell generated an open circuit voltage of 0.46 V, short circuit current density of 0.444 mA/cm 2 , and maximum power density of 0.062 mW/cm 2 at 0.26 V in the presence of 7 mM physiologic glucose. Upon device integration of the abiotic glucose hybrid fuel cell with the NFC module, the data from the glucose monitoring system is successfully transmitted to an android application for visualization at the user interface. The cell voltage correlated (r 2  = 0.989) with glucose concentration (up to 19 mM) with a sensitivity of 13.9 mV/mM•cm 2 . 
    more » « less
  • Citation Formats
  • MLA
  • APA
  • Chicago
  • BibTeX
  • Save / Share this Record
  • Send to Email