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  • Chromosome-level subgenome-aware de novo assembly provides insight into Saccharomyces bayanus genome divergence after hybridization
Title: Chromosome-level subgenome-aware de novo assembly provides insight into Saccharomyces bayanus genome divergence after hybridization
Interspecies hybridization is prevalent in various eukaryotic lineages and plays important roles in phenotypic diversification, adaptation, and speciation. To better understand the changes that occurred in the different subgenomes of a hybrid species and how they facilitate adaptation, we have completed chromosome-level de novo assemblies of all chromosomes for a recently formed hybrid yeast,Saccharomyces bayanusstrain CBS380, using Oxford Nanopore Technologies' MinION long-read sequencing. We characterize theS. bayanusgenome and compare it with its parent species,Saccharomyces uvarumandSaccharomyces eubayanus, and otherS. bayanusgenomes to better understand genome evolution after a relatively recent hybridization event. We observe multiple recombination events between the subgenomes in each chromosome, followed by loss of heterozygosity (LOH) in nine chromosome pairs. In addition to maintaining nearly all gene content and synteny from its parental genomes,S. bayanushas acquired many genes from other yeast species, primarily through the introgression ofSaccharomyces cerevisiae, such as those involved in the maltose metabolism. Finally, the patterns of recombination and LOH suggest an allotetraploid origin ofS. bayanus. The gene acquisition and rapid LOH in the hybrid genome probably facilitated its adaptation to maltose brewing environments and mitigated the maladaptive effect of hybridization. This paper describes the first in-depth study using long-read sequencing technology of anS. bayanushybrid genome, which may serve as an excellent reference for future studies of this important yeast and other yeast strains.  more » « less
Award ID(s):
1951332
PAR ID:
10596868
Author(s) / Creator(s):
; ; ; ; ; ; ; ;
Publisher / Repository:
Cold Spring Harbor Laboratory Press
Date Published:
Journal Name:
Genome Research
Volume:
34
Issue:
11
ISSN:
1088-9051
Page Range / eLocation ID:
2133 to 2146
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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