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Conference Title: 2021 ACM/IEEE Joint Conference on Digital Libraries (JCDL) Conference Start Date: 2021, Sept. 27 Conference End Date: 2021, Sept. 30 Conference Location: Champaign, IL, USAMetadata are key descriptors of research data, particularly for researchers seeking to apply machine learning (ML) to the vast collections of digitized specimens. Unfortunately, the available metadata is often sparse and, at times, erroneous. Additionally, it is prohibitively expensive to address these limitations through traditional, manual means. This paper reports on research that applies machine-driven approaches to analyzing digitized fish images and extracting various important features from them. The digitized fish specimens are being analyzed as part of the Biology Guided Neural Networks (BGNN) initiative, which is developing a novel class of artificial neural networks using phylogenies and anatomy ontologies. Automatically generated metadata is crucial for identifying the high-quality images needed for the neural network's predictive analytics. Methods that combine ML and image informatics techniques allow us to rapidly enrich the existing metadata associated with the 7,244 images from the Illinois Natural History Survey (INHS) used in our study. Results show we can accurately generate many key metadata properties relevant to the BGNN project, as well as general image quality metrics (e.g. brightness and contrast). Results also show that we can accurately generate bounding boxes and segmentation masks for fish, which are needed for subsequent machine learning analyses. The automatic process outperforms humans in terms of time and accuracy, and provides a novel solution for leveraging digitized specimens in ML. This research demonstrates the ability of computational methods to enhance the digital library services associated with the tens of thousands of digitized specimens stored in open-access repositories worldwide.
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On Image Quality Metadata, FAIR in ML, AI-Readiness and Reproducibility: Fish-AIR example
A new science discipline has emerged within the last decade at the intersection of informatics, computer science and biology:Imageomics. Like most other -omics fields, Imageomics also uses emerging technologies to analyze biological data but from the images. One of the most applied data analysis methods for image datasets is Machine Learning (ML). In 2019, we started working on a United States National Science Foundation (NSF) funded project, known as Biology Guided Neural Networks (BGNN) with the purpose of extracting information about biology by using neural networks and biological guidance such as species descriptions, identifications, phylogenetic trees and morphological annotations (Bart et al. 2021). Even though the variety and abundance of biological data is satisfactory for some ML analysis and the data are openly accessible, researchers still spend up to 80% of their time preparing data into a usable, AI-ready format, leaving only 20% for exploration and modeling (Long and Romanoff 2023). For this reason, we have built a dataset composed of digitized fish specimens, taken either directly from collections or from specialized repositories. The range of digital representations we cover is broad and growing, from photographs and radiographs, to CT scans, and even illustrations. We have added new groups of vocabularies to the dataset management system including image quality metadata, extended image metadata and batch metadata. With the image quality metadata and extended image metadata, we aimed to extract information from the digital objects that can possibly help ML scientists in their research with filtering, image processing and object recognition routines. Image quality metadata provides information about objects contained in the image, features and condition of the specimen, and some basic visual properties of the image, while extended image metadata provides information about technical properties of the digital file and the digital multimedia object (Bakış et al. 2021, Karnani et al. 2022, Leipzig et al. 2021, Pepper et al. 2021, Wang et al. 2021) (see details on Fish-AIR vocabulary web page). Batch metadata is used for separating different datasets and facilitates downloading and uploading data in batches with additional batch information and supplementary files. Additional flexibility, built into the database infrastructure using an RDF framework, will enable the system to host different taxonomic groups, which might require new metadata features (Jebbia et al. 2023). By the combination of these features, along with FAIR (Findable, Accessable, Interoperable, Reusable) principles, and reproducibility, we provide Artificial Intelligence Readiness (AIR; Long and Romanoff 2023) to the dataset. Fish-AIR provides an easy-to-access, filtered, annotated and cleaned biological dataset for researchers from different backgrounds and facilitates the integration of biological knowledge based on digitized preserved specimens into ML pipelines. Because of the flexible database infrastructure and addition of new datasets, researchers will also be able to access additional types of data—such as landmarks, specimen outlines, annotated parts, and quality scores—in the near future. Already, the dataset is the largest and most detailed AI-ready fish image dataset with integrated Image Quality Management System (Jebbia et al. 2023, Wang et al. 2021).
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- Award ID(s):
- 2118240
- PAR ID:
- 10612990
- Publisher / Repository:
- Sofia
- Date Published:
- Journal Name:
- Biodiversity Information Science and Standards
- Volume:
- 7
- ISSN:
- 2535-0897
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Adam, N.; Neuhold, E.; Furuta, R. (Ed.)Metadata is a key data source for researchers seeking to apply machine learning (ML) to the vast collections of digitized biological specimens that can be found online. Unfortunately, the associated metadata is often sparse and, at times, erroneous. This paper extends previous research conducted with the Illinois Natural History Survey (INHS) collection (7244 specimen images) that uses computational approaches to analyze image quality, and then automatically generates 22 metadata properties representing the image quality and morphological features of the specimens. In the research reported here, we demonstrate the extension of our initial work to University of the Wisconsin Zoological Museum (UWZM) collection (4155 specimen images). Further, we enhance our computational methods in four ways: (1) augmenting the training set, (2) applying contrast enhancement, (3) upscaling small objects, and (4) refining our processing logic. Together these new methods improved our overall error rates from 4.6 to 1.1%. These enhancements also allowed us to compute an additional set of 17 image-based metadata properties. The new metadata properties provide supplemental features and information that may also be used to analyze and classify the fish specimens. Examples of these new features include convex area, eccentricity, perimeter, skew, etc. The newly refined process further outperforms humans in terms of time and labor cost, as well as accuracy, providing a novel solution for leveraging digitized specimens with ML. This research demonstrates the ability of computational methods to enhance the digital library services associated with the tens of thousands of digitized specimens stored in open-access repositories world-wide by generating accurate and valuable metadata for those repositories.more » « less
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We have been successfully developing Artificial Intelligence (AI) models for automatically classifying fish species using neural networks over the last three years during the “Biology Guided Neural Network” (BGNN) project*1. We continue our efforts in another broader project, “Imageomics: A New Frontier of Biological Information Powered by Knowledge-Guided Machine Learning”*2. One of the main topics in the Imageomics Project is “Morphological Barcoding”. Within the Morphological Barcoding study, we are trying to build a gold standard method to identify species in different taxonomic groups based on their external morphology. This list of characters will contain, but not be limited to, landmarks, quantitative traits such as measurements of distances, areas, angles, proportions, colors, histograms, patterns, shapes, and outlines. The taxonomic groups will be limited by the data available, and we will be using fish as the topic of interest in this preliminary study. In this current study, we have focused on extracting morphological characters that are relying on anatomical features of fish, such as location of the eye, body length, and area of the head. We developed a schematic workflow to describe how we processed the data and extract the information (Fig. 1). We performed our analysis on the segmented images produced by Karpatne Team within the BGNN project (Bart et al. 2021). Segmentation analysis was performed using Artificial Neural Networks - Semantic Segmentation (Long et al. 2015); the list of segments to be detected were given as eye, head, trunk, caudal fin, pectoral fin, dorsal fin, anal fin, pelvic fin for fish. Segmented images, metadata and species lists were given as input to the workflow. During the cleaning and filtering subroutines, a subset of data was created by filtering down to the desired segmented images with corresponding metadata. In the validation step, segmented images were checked by comparing the number of specimens in the original image to the separate bounding-boxed specimen images, noting: violations in the segmentations, counts of segments, comparisons of relative positions of the segments among one another, traces of batch effect; comparisons according to their size and shape and finally based on these validation criteria each segmented image was assigned a score from 1 to 5 similar to Adobe XMP Basic namespace. The landmarks and the traits to be used in the study were extracted from the current literature, while mindful that some of the features may not be extracted successfully computationally. By using the landmark list, landmarks have been extracted by adapting the descriptions from the literature on to the segments, such as picking the left most point on the head as the tip of snout and top left point on the pelvic fin as base of the pelvic fin. 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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.3897/biss.7.112178
