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Abstract Phylogenomic data from a rapidly increasing number of studies provide new evidence for resolving relationships in recently radiated clades, but they also pose new challenges for inferring evolutionary histories. Most existing methods for reconstructing phylogenetic hypotheses rely solely on algorithms that only consider incomplete lineage sorting (ILS) as a cause of intra- or intergenomic discordance. Here, we utilize a variety of methods, including those to infer phylogenetic networks, to account for both ILS and introgression as a cause for nuclear and cytoplasmic-nuclear discordance using phylogenomic data from the recently radiated flowering plant genus Polemonium (Polemoniaceae), an ecologically diverse genus in Western North America with known and suspected gene flow between species. We find evidence for widespread discordance among nuclear loci that can be explained by both ILS and reticulate evolution in the evolutionary history of Polemonium. Furthermore, the histories of organellar genomes show strong discordance with the inferred species tree from the nuclear genome. Discordance between the nuclear and plastid genome is not completely explained by ILS, and only one case of discordance is explained by detected introgression events. Our results suggest that multiple processes have been involved in the evolutionary history of Polemonium and that the plastid genome does not accurately reflect species relationships. We discuss several potential causes for this cytoplasmic-nuclear discordance, which emerging evidence suggests is more widespread across the Tree of Life than previously thought. [Cyto-nuclear discordance, genomic discordance, phylogenetic networks, plastid capture, Polemoniaceae, Polemonium, reticulations.]
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Resolving phylogenetic relationships of a recent and rapidly evolving clade from western North America (Mentzelia section Bartonia, Loasaceae)
The landscape of western North America has dramatically transformed since the Miocene to become increasingly heterogeneous, in turn promoting the evolution of many rapidly radiating angiosperm lineages. Phylogenetic relationships of these recently and rapidly radiating groups are difficult to resolve as there is little genetic variation among species and a high degree of noise from incomplete lineage sorting and hybridization. Mentzelia section Bartonia (51 species; Loasaceae) exemplifies this problem well. The clade has been investigated with Sanger sequencing, RADSeq, and genome skimming methods, however, most species relationships remain elusive due to low genetic variability. To better infer species relationships, we applied a hybrid enrichment approach with the Angiosperms353 probe set and implemented a novel bioinformatics workflow that aimed to maximize phylogenetic signal and minimize noise from low-quality sequences, paralogy, and incomplete lineage sorting. Our phylogenomic approach increased phylogenetic resolution of species relationships compared to previous studies based on nrDNA loci. Although a few species relationships still lack strong support, our results indicate that our methods were effective in phylogenetic inference of this recently and rapidly evolving lineage from western North America. To better characterize major groups in the Section, we propose the formal designation of three subsections: Decapetala, Multicaulis, and Multiflora.
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- Award ID(s):
- 2117446
- PAR ID:
- 10633022
- Publisher / Repository:
- Systematic Botany
- Date Published:
- Volume:
- 50
- Issue:
- 1
- Page Range / eLocation ID:
- 67-82
- Subject(s) / Keyword(s):
- Angiosperms353, bioinformatics pipeline, high throughput sequencing, HybPiper, phylogenomics, rapid radiation.
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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