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1. Triple Oxygen Isotope Measurements (Δ'17O) of Body Water Reflect Water Intake, Metabolism, and δ18O of Ingested Water in Passerines

   https://doi.org/10.3389/fphys.2021.710026  

   Sabat, Pablo; Newsome, Seth D.; Pinochet, Stephanie; Nespolo, Roberto; Sanchez-Hernandez, Juan Carlos; Maldonado, Karin; Gerson, Alexander R.; Sharp, Zachary D.; Whiteman, John P. (September 2021, Frontiers in Physiology)

   Understanding physiological traits and ecological conditions that influence a species reliance on metabolic water is critical to creating accurate physiological models that can assess their ability to adapt to environmental perturbations (e.g., drought) that impact water availability. However, relatively few studies have examined variation in the sources of water animals use to maintain water balance, and even fewer have focused on the role of metabolic water. A key reason is methodological limitations. Here, we applied a new method that measures the triple oxygen isotopic composition of a single blood sample to estimate the contribution of metabolic water to the body water pool of three passerine species. This approach relies on Δ' 17 O, defined as the residual from the tight linear correlation that naturally exists between δ 17 O and δ 18 O values. Importantly, Δ'17O is relatively insensitive to key fractionation processes, such as Rayleigh distillation in the water cycle that have hindered previous isotope-based assessments of animal water balance. We evaluated the effects of changes in metabolic rate and water intake on Δ' 17 O values of captive rufous-collared sparrows ( Zonotrichia capensis ) and two invertivorous passerine species in the genus Cinclodes from the field. As predicted, colder acclimation temperatures induced increases in metabolic rate, decreases in water intake, and increases in the contribution of metabolic water to the body water pool of Z. capensis , causing a consistent change in Δ' 17 O. Measurement of Δ' 17 O also provides an estimate of the δ 18 O composition of ingested pre-formed (drinking/food) water. Estimated δ 18 O values of drinking/food water for captive Z. capensis were ~ −11‰, which is consistent with that of tap water in Santiago, Chile. In contrast, δ 18 O values of drinking/food water ingested by wild-caught Cinclodes were similar to that of seawater, which is consistent with their reliance on marine resources. Our results confirm the utility of this method for quantifying the relative contribution of metabolic versus pre-formed drinking/food water to the body water pool in birds. 

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2. A multi-isotope approach reveals seasonal variation in the reliance on marine resources, production of metabolic water, and ingestion of seawater by two species of coastal passerine to maintain water balance

   https://doi.org/10.3389/fevo.2023.1120271  

   Navarrete, Lucas; Lübcker, Nico; Alvarez, Felipe; Nespolo, Roberto; Sanchez-Hernandez, Juan Carlos; Maldonado, Karin; Sharp, Zachary D; Whiteman, John P; Newsome, Seth D; Sabat, Pablo (February 2023, Frontiers in Ecology and Evolution)

   Tracing how free-ranging organisms interact with their environment to maintain water balance is a difficult topic to study for logistical and methodological reasons. We use a novel combination of triple-oxygen stable isotope analyses of water extracted from plasma (δ¹⁶O, δ¹⁷O, δ¹⁸O) and bulk tissue carbon (δ¹³C) and nitrogen (δ¹⁵N) isotopes of feathers and blood to estimate the proportional contribution of marine resources, seawater, and metabolic water used by two species of unique songbirds (genusCinclodes) to maintain their water balance in a seasonal coastal environment. We also assessed the physiological adjustments that these birds use to maintain their water balance. In agreement with previous work on these species, δ¹³C and δ¹⁵N data show that the coastal resident and invertivoreC. nigrofumosusconsumes a diet rich in marine resources, while the diet of migratoryC. oustaletishifts seasonally between marine (winter) to freshwater aquatic resources (summer). Triple-oxygen isotope analysis (Δ¹⁷O) of blood plasma, basal metabolic rate (BMR), and total evaporative water loss (TEWL) revealed that ~25% of the body water pool of both species originated from metabolic water, while the rest originated from a mix of seawater and fresh water. Δ¹⁷O measurements suggest that the contribution of metabolic water tends to increase in summer inC. nigrofumosus, which is coupled with a significant increase in BMR and TEWL. The two species had similar BMR and TEWL during the austral winter when they occur sympatrically in coastal environments. We also found a positive and significant association between the use of marine resources as measured by δ¹³C and δ¹⁵N values and the estimated δ¹⁸O values of ingested (pre-formed) water in both species, which indicates that Cinclodes do not directly drink seawater but rather passively ingest when consuming marine invertebrates. Finally, results obtained from physiological parameters and the isotope-based estimates of marine (food and water) resource use are consistent, supporting the use of the triple-oxygen isotopes to quantify the contribution of water sources to the total water balance of free-ranging birds. 

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3. Seasonal SIMS δ18O record in Astarte borealis from the Baltic Sea tracks a modern regime shift in the NAO

   https://doi.org/10.3389/fmars.2023.1293823  

   Hughes, Hunter P; Surge, Donna; Orland, Ian J; Zettler, Michael L; Moss, David K (December 2023, Frontiers in Marine Science)

   IntroductionAstarte borealisholds great potential as an archive of seasonal paleoclimate, especially due to its long lifespan (several decades to more than a century) and ubiquitous distribution across high northern latitudes. Furthermore, recent work demonstrates that the isotope geochemistry of the aragonite shell is a faithful proxy of environmental conditions. However, the exceedingly slow growth rates ofA. borealisin some locations (<0.2mm/year) make it difficult to achieve seasonal resolution using standard micromilling techniques for conventional stable isotope analysis. Moreover, oxygen isotope (δ¹⁸O) records from species inhabiting brackish environments are notoriously difficult to use as paleoclimate archives because of the simultaneous variation in temperature and δ¹⁸O_watervalues. MethodsHere we use secondary ion mass spectrometry (SIMS) to microsample anA. borealisspecimen from the southern Baltic Sea, yielding 451 SIMS δ¹⁸O_shellvalues at sub-monthly resolution. ResultsSIMS δ¹⁸O_shellvalues exhibit a quasi-sinusoidal pattern with 24 local maxima and minima coinciding with 24 annual growth increments between March 1977 and the month before specimen collection in May 2001. DiscussionAge-modeled SIMS δ¹⁸O_shellvalues correlate significantly with bothin situtemperature measured from shipborne CTD casts (r² = 0.52, p<0.001) and sea surface temperature from the ORAS5-SST global reanalysis product for the Baltic Sea region (r² = 0.42, p<0.001). We observe the strongest correlation between SIMS δ¹⁸O_shellvalues and salinity when both datasets are run through a 36-month LOWESS function (r² = 0.71, p < 0.001). Similarly, we find that LOWESS-smoothed SIMS δ¹⁸O_shellvalues exhibit a moderate correlation with the LOWESS-smoothed North Atlantic Oscillation (NAO) Index (r² = 0.46, p<0.001). Change point analysis supports that SIMS δ¹⁸O_shellvalues capture a well-documented regime shift in the NAO circa 1989. We hypothesize that the correlation between the SIMS δ¹⁸O_shelltime series and the NAO is enhanced by the latter’s influence on the regional covariance of water temperature and δ¹⁸O_watervalues on interannual and longer timescales in the Baltic Sea. These results showcase the potential for SIMS δ¹⁸O_shellvalues inA. borealisshells to provide robust paleoclimate information regarding hydroclimate variability from seasonal to decadal timescales. 

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4. HfO ₂ -based platform for high-index-contrast visible and UV integrated photonics

   https://doi.org/10.1364/OL.553552  

   Jaramillo, Oscar; Natarajan, Vighnesh; Mahmud_Rivy, Hamim; Tensuan, Joshua; Massai, Leonardo; Mehta, Karan K (January 2025, Optics Letters)

   Ultraviolet and visible integrated photonics enable applications in quantum information, sensing, and spectroscopy, among others. Few materials support low-loss photonics into the UV, and the relatively low refractive index of known depositable materials limits the achievable functionality. Here, we present a high-index integrated photonics platform based on HfO₂and Al₂O₃composites deposited via atomic layer deposition (ALD) with low loss in the visible and near UV. We show that Al₂O₃incorporation dramatically decreases bulk loss compared to pure HfO₂, consistent with inhibited crystallization due to the admixture of Al₂O₃. Composites exhibit refractive indexnfollowing the average of that of HfO₂and Al₂O₃, weighted by the HfO₂fractional compositionx. Atλ = 375 nm, composites withx = 0.67 exhibitn = 2.01, preserving most of HfO₂’s significantly higher index, and 3.8(7) dB/cm material loss. We further present fully etched and cladded waveguides, grating couplers, and ring resonators, realizing a single-mode waveguide loss of 0.25(2) dB/cm inferred from resonators of 2.6 million intrinsic quality factor atλ = 729 nm, 2.6(2) dB/cm atλ = 405 nm, and 7.7(6) dB/cm atλ = 375 nm. We measure the composite’s thermo-optic coefficient (TOC) to be 2.44(3) × 10⁻⁵RIU/°C nearλ = 397 nm. This work establishes (HfO₂)_x(Al₂O₃)_1−xcomposites as a platform amenable to integration for low-loss, high-index photonics spanning the UV to NIR. 

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5. In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance

   https://doi.org/10.1073/pnas.2505083122  

   McDonald, Julie L; Shapiro, Nathan P; Mengiste, Amanuella A; Kaines, Sarah; Whitney, Spencer M; Wilson, Robert H; Shoulders, Matthew D (July 2025, Proceedings of the National Academy of Sciences)

   Carbon dioxide (CO₂) assimilation by the enzyme Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) underpins biomass accumulation in photosynthetic bacteria and eukaryotes. Despite its pivotal role, Rubisco has a slow carboxylation rate ( $k_{\mathit{cat}}^{{\mathit{CO}}_2}$) and is competitively inhibited by oxygen (O₂). These traits impose limitations on photosynthetic efficiency, making Rubisco a compelling target for improvement. Interest in Form II Rubisco fromGallionellaceaebacteria, which comprise a dimer or hexamer of large subunits, arises from their nearly fivefold higher $k_{\mathit{cat}}^{{\mathit{CO}}_2}$than the average Rubisco enzyme. As well as having a fast $k_{\mathit{cat}}^{{\mathit{CO}}_2}$(25.8 s⁻¹at 25 °C), we show thatGallionellaceaeRubisco (GWS1B) is extremely sensitive to O₂inhibition, consistent with its evolution under semianaerobic environments. We therefore used an in vivo mutagenesis-mediated screening pipeline to evolve GWS1B over six rounds under oxygenic selection, identifying three catalytic point mutants with improved ambient carboxylation efficiency: Thr-29-Ala (T29A), Glu-40-Lys (E40K), and Arg-337-Cys (R337C). Full kinetic characterization showed that each substitution enhanced the CO₂affinity of GWS1B under oxygenic conditions by subduing oxygen affinity, leading to 25% (E40K), 11% (T29A), and 8% (R337C) enhancements in carboxylation efficiency under ambient O₂at 25 °C. By contrast, under the near anaerobic natural environment ofGallionellaceae, the carboxylation efficiency of each mutant was impaired ~16%. These findings demonstrate the efficacy of artificial directed evolution to access distinctive regions of catalytic space in Rubisco. 

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