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Abstract Although long-read single-cell RNA isoform sequencing (scISO-Seq) can reveal alternative RNA splicing in individual cells, it suffers from a low read throughput. Here, we introduce HIT-scISOseq, a method that removes most artifact cDNAs and concatenates multiple cDNAs for PacBio circular consensus sequencing (CCS) to achieve high-throughput and high-accuracy single-cell RNA isoform sequencing. HIT-scISOseq can yield >10 million high-accuracy long-reads in a single PacBio Sequel II SMRT Cell 8M. We also report the development of scISA-Tools that demultiplex HIT-scISOseq concatenated reads into single-cell cDNA reads with >99.99% accuracy and specificity. We apply HIT-scISOseq to characterize the transcriptomes of 3375 corneal limbus cells and reveal cell-type-specific isoform expression in them. HIT-scISOseq is a high-throughput, high-accuracy, technically accessible method and it can accelerate the burgeoning field of long-read single-cell transcriptomics.
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This content will become publicly available on July 8, 2026
Iohexol as a refractive index tuning agent for bioinks in high cell density bioprinting
An iohexol-based bioink enables high-resolution, high cell density 3D bioprinting by reducing light scattering through refractive index matching, supporting cell viability and perfusable tissue fabrication.
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- Award ID(s):
- 2135720
- PAR ID:
- 10659920
- Publisher / Repository:
- The Royal Society of Chemistry
- Date Published:
- Journal Name:
- Biomaterials Science
- Volume:
- 13
- Issue:
- 14
- ISSN:
- 2047-4830
- Page Range / eLocation ID:
- 3958 to 3971
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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