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This content will become publicly available on February 1, 2027

Title: Peroxidase-mimicking Ce–hemin–MOFs/GO composite for enzyme-free electrochemical detection of H2O2
Hydrogen peroxide (H2O2), a key reactive oxygen species (ROS), plays a crucial role in cellular signaling; however, at elevated concentrations, it contributes to oxidative stress and is implicated in various pathologies. Herein, we report the development of a novel electrochemical biosensor based on cerium–hemin metal–organic frameworks (Ce–hemin–MOFs ) integrated with graphene oxide (GO) for the sensitive and selective detection of H2O2. The Ce–hemin–MOFs were synthesized via a coordination-driven assembly of cerium ions and hemin, yielding petal-like crystalline microstructures with intrinsic peroxidase-mimicking activity. Incorporation of GO significantly enhanced the electrical conductivity of the composite. The sensor demonstrated a broad linear detection range (0.01–10 mM), a low detection limit of 1.2 μM, and strong selectivity against common biological interferents. Furthermore, the developed sensor enabled real-time detection of H2O2 released from human prostate cancer (22Rv1) cells, demonstrating its practical potential for monitoring oxidative processes associated with cellular pathophysiology. This highlights the broader applicability of MOFs-based sensing platforms in biomedical research and disease diagnostics.  more » « less
Award ID(s):
2141183
PAR ID:
10659925
Author(s) / Creator(s):
; ;
Publisher / Repository:
Elsevier
Date Published:
Journal Name:
Journal of Electroanalytical Chemistry
Volume:
1002
Issue:
C
ISSN:
1572-6657
Page Range / eLocation ID:
119744
Subject(s) / Keyword(s):
Electrochemical sensor, Nanozyme, Metal–organic frameworks (MOFs), Cellular oxidative stress, Hydrogen peroxide (H2O2)
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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