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The characterization of drug-target interactions is a key component of drug discovery, testing, and development. Affinity chromatography is one approach that can be used for this type of analysis. For instance, this may be done by using an immobilized target as a stationary phase and a drug as the applied solute. This review will discuss the various ways in which affinity chromatographic methods have been used to examine drug-target interactions, with an emphasis on high-performance methods. The general principles of this approach and factors to consider in its use for drug-target interaction analysis will first be examined. Methods based on zonal elution or frontal analysis for binding and competition studies will then be discussed. Various techniques for kinetic studies will next be considered, along with approaches that employ secondary binding agents and hybrid techniques. In each case, the general principles and theory of an approach will be given along with examples of its use in drug-target interaction studies. Advantages or limitations of each approach will be provided as well. This information should make it possible in the future to extend these techniques to other drug-target systems of interest in biomedical research and drug testing or development.
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Microscale Affinity Chromatography for Biointeraction Analysis: Strategies, Principles and Applications
The analysis of interactions between biological agents or with surrounding chemicals is important in many areas of modern biochemical, biomedical, and environmental research. Microscale platforms based on affinity chromatography have been shown to be a powerful set of tools for these studies. This approach makes use of an immobilized binding agent as a stationary phase in a microscale platform for either direct examination of the interactions of this agent with an applied target solute or as a secondary capture agent to probe a solution‐phase interaction. This review will examine the various platforms and strategies that have been used in microscale affinity chromatography, or µAC, to characterize and study biointeractions. The general principles of µAC and schemes based on this approach will be examined, along with applications of this technique. Examples of approaches that will be considered will include zonal and frontal analysis methods, as well as a variety of schemes by which µAC can be employed in kinetic studies. In each case, the theory and principles of these methods will be provided along with examples of their use in biointeraction studies.
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- PAR ID:
- 10668283
- Publisher / Repository:
- Wiley
- Date Published:
- Journal Name:
- Journal of Separation Science
- Volume:
- 49
- Issue:
- 2
- ISSN:
- 1615-9306
- Subject(s) / Keyword(s):
- Microscale affinity chromatography Affinity microcolumn Biointeraction analysis Binding studies Kinetic studies
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript
- Journal Article:
- https://doi.org/10.1002/jssc.70361
