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Transmissible cancers are infectious parasitic clones that metastasize to new hosts, living past the death of the founder animal in which the cancer initiated. We investigated the evolutionary history of a cancer lineage that has spread though the soft-shell clam (Mya arenaria) population by assembling a chromosome-scale soft-shell clam reference genome and characterizing somatic mutations in transmissible cancer. We observe high mutation density, widespread copy-number gain, structural rearrangement, loss of heterozygosity, variable telomere lengths, mitochondrial genome expansion and transposable element activity, all indicative of an unstable cancer genome. We also discover a previously unreported mutational signature associated with overexpression of an error-prone polymerase and use this to estimate the lineage to be >200 years old. Our study reveals the ability for an invertebrate cancer lineage to survive for centuries while its genome continues to structurally mutate, likely contributing to the evolution of this lineage as a parasitic cancer.

 

Abstract We use the results of a survey for low-surface-gravity stars in Galactic (and LMC) globular clusters to show that “yellow” post-asymptotic-branch (yPAGB) stars are likely to be excellent extragalactic standard candles, capable of producing distances to early-type galaxies that are accurate to a couple of percent. We show that the mean bolometric magnitude of the 10 yPAGB stars in globular clusters is 〈 M bol 〉 = −3.38 ± 0.03, a value that is ∼0.2 mag brighter than that predicted from the latest post-horizontal-branch evolutionary tracks. More importantly, we show that the observed dispersion in the distribution is only 0.10 mag, i.e., better than the scatter for individual Cepheids. We describe the physics that can produce such a small dispersion and show that, if one restricts surveys to the color range 0.0 ≲ ( B − V ) 0 ≲ 0.5, then samples of nonvariable yPAGB stars can be identified quite easily with a minimum of contamination. The extremely bright absolute V magnitudes of these stars (〈 M V 〉 = −3.37) make them, by far, the visually brightest objects in old stellar populations and ideal Population II standard candles for measurements out to ∼10 Mpc with current instrumentation. A Hubble Space Telescope survey in the halos of galaxies in the M81 and Sculptor groups could therefore serve as an effective cross-check on both the Cepheid and tip-of-the-red-giant-branch distance scales. 

Abstract We have carried out a search for above-horizontal-branch (AHB) stars—objects lying above the horizontal branch (HB) and blueward of the asymptotic giant branch (AGB) in the color–magnitude diagram—in 97 Galactic and seven Magellanic Cloud globular clusters (GCs). We selected AHB candidates based on photometry in the uBVI system, which is optimized for detection of low-surface-gravity stars with large Balmer jumps, in the color range −0.05 ≤ ( B − V ) 0 ≤1.0. We then used Gaia astrometry and Gaussian-mixture modeling to confirm cluster membership and remove field interlopers. Our final catalog contains 438 AHB stars, classified and interpreted in the context of post-HB evolution as follows: (1) AHB1: 280 stars fainter than M V = −0.8, evolving redward from the blue HB (BHB) toward the base of the AGB. (2) Post-AGB (PAGB): 13 stars brighter than M V ≃ −2.75, departing from the top of the AGB and evolving rapidly blueward. (3) AHB2: 145 stars, with absolute magnitudes between those of the AHB1 and PAGB groups. This last category includes a mixture of objects leaving the extreme BHB and evolving toward the AGB, and brighter ones moving back from the AGB toward higher temperatures. Among the AHB1 stars are 59 RR Lyrae interlopers, observed by chance in our survey near maximum light. PAGB and AHB2 stars (including W Virginis Cepheids) overwhelmingly belong to GCs containing BHB stars, in accordance with predictions of post-HB evolutionary tracks. We suggest that most W Vir variables are evolving toward lower temperatures and are in their first crossings of the instability strip. Nonvariable yellow PAGB stars show promise as a Population II standard candle for distance measurement. 

Canine distemper virus (CDV) is a multi-host pathogen with variable clinical outcomes of infection across and within species. We used whole-genome sequencing (WGS) to search for viral markers correlated with clinical distemper in African lions. To identify candidate markers, we first documented single-nucleotide polymorphisms (SNPs) differentiating CDV strains associated with different clinical outcomes in lions in East Africa. We then conducted evolutionary analyses on WGS from all global CDV lineages to identify loci subject to selection. SNPs that both differentiated East African strains and were under selection were mapped to a phylogenetic tree representing global CDV diversity to assess if candidate markers correlated with documented outbreaks of clinical distemper in lions (n = 3). Of 54 SNPs differentiating East African strains, ten were under positive or episodic diversifying selection and 20 occurred in the clinical strain despite strong purifying selection at those loci. Candidate markers were in functional domains of the RNP complex (n = 19), the matrix protein (n = 4), on CDV glycoproteins (n = 5), and on the V protein (n = 1). We found mutations at two loci in common between sequences from three CDV outbreaks of clinical distemper in African lions; one in the signaling lymphocytic activation molecule receptor (SLAM)-binding region of the hemagglutinin protein and another in the catalytic center of phosphodiester bond formation on the large polymerase protein. These results suggest convergent evolution at these sites may have a functional role in clinical distemper outbreaks in African lions and uncover potential novel barriers to pathogenicity in this species. 

Numerous pairs of evolutionarily divergent mammalian species have been shown to produce hybrid offspring. In some cases, F 1 hybrids are able to produce F 2 s through matings with F 1 s. In other instances, the hybrids are only able to produce offspring themselves through backcrosses with a parent species owing to unisexual sterility (Haldane's Rule). Here, we explicitly tested whether genetic distance, computed from mitochondrial and nuclear genes, can be used as a proxy to predict the relative fertility of the hybrid offspring resulting from matings between species of terrestrial mammals. We assessed the proxy's predictive power using a well-characterized felid hybrid system, and applied it to modern and ancient hominins. Our results revealed a small overlap in mitochondrial genetic distance values that distinguish species pairs whose calculated distances fall within two categories: those whose hybrid offspring follow Haldane's Rule, and those whose hybrid F 1 offspring can produce F 2 s. The strong correlation between genetic distance and hybrid fertility demonstrated here suggests that this proxy can be employed to predict whether the hybrid offspring of two mammalian species will follow Haldane's Rule. 

The outcome of pathogen spillover from a reservoir to a novel host population can range from a “dead‐end” when there is no onward transmission in the recipient population, to epidemic spread and even establishment in new hosts. Understanding the evolutionary epidemiology of spillover events leading to discrete outcomes in novel hosts is key to predicting risk and can lead to a better understanding of the mechanisms of emergence. Here we use a Bayesian phylodynamic approach to examine cross‐species transmission and evolutionary dynamics during a canine distemper virus (CDV) spillover event causing clinical disease and population decline in an African lion population (Panthera leo) in the Serengeti Ecological Region between 1993 and 1994. Using 21 near‐complete viral genomes from four species we found that this large‐scale outbreak was likely  ignited by a single cross‐species spillover event from a canid reservoir to noncanid hosts <1 year before disease detection and explosive spread of CDV in lions. Cross‐species transmission from other noncanid species probably fuelled the high prevalence of CDV across spatially structured lion prides. Multiple lines of evidence suggest that spotted hyenas (Crocuta crocuta) could have acted as the proximate source of CDV exposure in lions. We report 13 nucleotide substitutions segregating CDV strains found in canids and noncanids. Our results are consistent with the hypothesis that virus evolution played a role in CDV emergence in noncanid hosts following spillover during the outbreak, suggest that host barriers to clinical infection can limit outcomes of CDV spillover in novel host species.