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Abstract Artificial intelligence (AI) has gained widespread public interest in recent years. However, as AI literacy remained excluded from the standard academic curricula, AI education in the US was predominantly offered through extra-curricular activities, which limited AI learning exposure to only a select group of students. Given these limitations, the need to integrate AI literacy education into the standard curricula is increasingly evident. This study investigated the integration of AI learning in an advanced biology course. Thirty-seven students participated in four lessons embedding AI learning in biology contexts. The interplay of students’ AI learning and biology knowledge was examined from the quantitative measure of conceptual understanding and qualitative analysis of interdisciplinary reasoning. This concurrent triangulation research design utilized results from both quantitative and qualitative analyses to develop a comprehensive understanding of students’ AI learning in the biology context. The results of the study showed a significant improvement in students’ AI concepts. Students’ biology knowledge had a slight increase, but it was not statistically significant. Both quantitative and qualitative results underscored a close connection between students’ AI learning and their biology knowledge, though the quantitative findings were not conclusive in some lessons. The article concluded with a discussion of the potential reasons for those discrepancies. In addition, suggestions were provided for future research and practitioners who are interested in integrating AI education across curricula. 

Abstract Colorectal cancer is one of the top contributors to cancer-related deaths in the United States, with over 100,000 estimated cases in 2020 and over 50,000 deaths. The most common screening technique is minimally invasive colonoscopy using either reflected white light endoscopy or narrow-band imaging. However, current imaging modalities have only moderate sensitivity and specificity for lesion detection. We have developed a novel fluorescence excitation-scanning hyperspectral imaging (HSI) approach to sample image and spectroscopic data simultaneously on microscope and endoscope platforms for enhanced diagnostic potential. Unfortunately, fluorescence excitation-scanning HSI datasets pose major challenges for data processing, interpretability, and classification due to their high dimensionality. Here, we present an end-to-end scalable Artificial Intelligence (AI) framework built for classification of excitation-scanning HSI microscopy data that provides accurate image classification and interpretability of the AI decision-making process. The developed AI framework is able to perform real-time HSI classification with different speed/classification performance trade-offs by tailoring the dimensionality of the dataset, supporting different dimensions of deep learning models, and varying the architecture of deep learning models. We have also incorporated tools to visualize the exact location of the lesion detected by the AI decision-making process and to provide heatmap-based pixel-by-pixel interpretability. In addition, our deep learning framework provides wavelength-dependent impact as a heatmap, which allows visualization of the contributions of HSI wavelength bands during the AI decision-making process. This framework is well-suited for HSI microscope and endoscope platforms, where real-time analysis and visualization of classification results are required by clinicians.