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As a supplementary or the only water source in dry regions, dew plays a critical role in the survival of organisms. The new hydrological tracer¹⁷O-excess, with almost sole dependence on relative humidity, provides a new way to distinguish the evaporation processes and reconstruct the paleoclimate. Up to now, there is no published daily dew isotope record on δ²H, δ¹⁸O, δ¹⁷O, d-excess, and¹⁷O-excess. Here, we collected daily dew between July 2014 and April 2018 from three distinct climatic regions (i.e., Gobabeb in the central Namib Desert with desert climate, Nice in France with Mediterranean climate, and Indianapolis in the central United States with humid continental climate). The δ²H, δ¹⁸O, and δ¹⁷O of dew were simultaneously analyzed using a Triple Water Vapor Isotope Analyzer based on Off-Axis Integrated Cavity Output Spectroscopy technique, and then d-excess and¹⁷O-excess were calculated. This report presents daily dew isotope dataset under three climatic regions. It is useful for researchers to use it as a reference when studying global dew dynamics and dew formation mechanisms.