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Point-of-Care Peptide Hormone Production Enabled by Cell-Free Protein Synthesis

https://doi.org/10.1021/acssynbio.2c00680

DeWinter, Madison A.; Thames, Ariel Helms; Guerrero, Laura; Kightlinger, Weston; Karim, Ashty S.; Jewett, Michael C. (April 2023, ACS Synthetic Biology)

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Cell-free systems for accelerating glycoprotein expression and biomanufacturing

https://doi.org/10.1007/s10295-020-02321-4

Hershewe, Jasmine; Kightlinger, Weston; Jewett, Michael C (November 2020, Journal of Industrial Microbiology and Biotechnology)
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Abstract Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar moieties, plays critical roles in cellular function, human health, and biotechnology. However, studying and producing defined glycoproteins remains challenging. Cell-free glycoprotein synthesis systems, in which protein synthesis and glycosylation are performed in crude cell extracts, offer new approaches to address these challenges. Here, we review versatile, state-of-the-art systems for biomanufacturing glycoproteins in prokaryotic and eukaryotic cell-free systems with natural and synthetic N-linked glycosylation pathways. We discuss existing challenges and future opportunities in the use of cell-free systems for the design, manufacture, and study of glycoprotein biomedicines.
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Synthetic Glycobiology: Parts, Systems, and Applications

https://doi.org/10.1021/acssynbio.0c00210

Kightlinger, Weston; Warfel, Katherine F.; DeLisa, Matthew P.; Jewett, Michael C. (July 2020, ACS Synthetic Biology)

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