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Abstract The ability to distinguish between the abdominal conditions of adult female mosquitoes has important utility for the surveillance and control of mosquito-borne diseases. However, doing so requires entomological training and time-consuming manual effort. Here, we design computer vision techniques to determine stages in the gonotrophic cycle of female mosquitoes from images. Our dataset was collected from 139 adult female mosquitoes across three medically important species—Aedes aegypti,Anopheles stephensi, andCulex quinquefasciatus—and all four gonotrophic stages of the cycle (unfed, fully fed, semi-gravid, and gravid). From these mosquitoes and stages, a total of 1959 images were captured on a plain background via multiple smartphones. Subsequently, we trained four distinct AI model architectures (ResNet50,MobileNetV2,EfficientNet-B0, andConvNeXtTiny), validated them using unseen data, and compared their overall classification accuracies. Additionally, we analyzed t-SNE plots to visualize the formation of decision boundaries in a lower-dimensional space. Notably,ResNet50andEfficientNet-B0demonstrated outstanding performance with an overall accuracy of 97.44% and 93.59%, respectively.EfficientNet-B0demonstrated the best overall performance considering computational efficiency, model size, training speed, and t-SNE decision boundaries. We also assessed the explainability of thisEfficientNet-B0model, by implementing Grad-CAMs—a technique that highlights pixels in an image that were prioritized for classification. We observed that the highest weight was for those pixels representing the mosquito abdomen, demonstrating that our AI model has indeed learned correctly. Our work has significant practical impact. First, image datasets for gonotrophic stages of mosquitoes are not yet available. Second, our algorithms can be integrated with existing citizen science platforms that enable the public to record and upload biological observations. With such integration, our algorithms will enable the public to contribute to mosquito surveillance and gonotrophic stage identification. Finally, we are aware of work today that uses computer vision techniques for automated mosquito species identification, and our algorithms in this paper can augment these efforts by enabling the automated detection of gonotrophic stages of mosquitoes as well. 

Strong interactions and topology drive a wide variety of correlated ground states. Some of the most interesting of these ground states, such as fractional quantum Hall states and fractional Chern insulators, have fractionally charged quasiparticles. Correlations in these phases are captured by the binding of electrons and vortices into emergent particles called composite fermions. Composite fermion quasiparticles are randomly localized at high levels of disorder and may exhibit charge order when there is not too much disorder in the system. However, more complex correlations are predicted when composite fermion quasiparticles cluster into a bubble, and then these bubbles order on a lattice. Such a highly correlated ground state is termed the bubble phase of composite fermions. Here we report the observation of such a bubble phase of composite fermions, evidenced by the re-entrance of the fractional quantum Hall effect. We associate this re-entrance with a bubble phase with two composite fermion quasiparticles per bubble. Our results demonstrate the existence of a new class of strongly correlated topological phases driven by clustering and charge ordering of emergent quasiparticles. 

Abstract This paper presents the genetic algorithm (GA) and particle swarm optimization (PSO) based frequency regulation for a wind‐based microgrid (MG) using reactive power balance loop. MG, operating from squirrel cage induction generator (SCIG), is employed for exporting the electrical power from wind turbines, and it needs reactive power which may be imported from the grid. Additional reactive power is also required from the grid for the load, directly coupled with such a distributed generator (DG) plant. However, guidelines issued by electric authorities encourage MGs to arrange their own reactive power because such reactive power procurement is defined as a local area problem for power system studies. Despite the higher cost of compensation, static synchronous compensator (STATCOM) is a fast‐acting FACTs device for attending to these reactive power mismatches. Reactive power control can be achieved by controlling reactive current through the STATCOM. This can be achieved with modification in current controller scheme of STATCOM. STATCOM current controller is designed with reactive power load balance for the proposed microgrid in this paper. Further, gain values of the PI controller, required in the STATCOM model, are selected first with classical methods. In this classical method, iterative procedures which are based on integral square error (ISE), integral absolute error (IAE), and integral square of time error (ISTE) criteria are developed using MATLAB programs. System performances are further investigated with GA and PSO based control techniques and their acceptability over classical methods is diagnosed. Results in terms of converter frequency deviation show how the frequency remains under the operating boundaries as allowed by IEEE standards 1159:1995 and 1250:2011 for integrating renewable‐based microgrid with grid. Real and reactive power management and load current total harmonic distortions verify the STATCOM performance in MG. The results are further validated with the help of recent papers in which frequency regulation is investigated for almost similar power system models. The compendium for this work is as following: (i) modelling of wind generator‐based microgrid using MATLAB simulink library, (ii) designing of STATCOM current controller with PI controller, (iii) gain constants estimation using classical, GA and PSO algorithm through a developed m codes and their interfacing with proposed simulink model, (v) dynamic frequency responses for proposed grid connected microgrid during starting and load perturbations, (vi) verification of system performance with the help of obtained real and reactive power management between STATCOM and grid, and (vii) validation of results with available literature. 

One of the most fundamental questions in ecology is how many species inhabit the Earth. However, due to massive logistical and financial challenges and taxonomic difficulties connected to the species concept definition, the global numbers of species, including those of important and well-studied life forms such as trees, still remain largely unknown. Here, based on global ground-sourced data, we estimate the total tree species richness at global, continental, and biome levels. Our results indicate that there are ∼73,000 tree species globally, among which ∼9,000 tree species are yet to be discovered. Roughly 40% of undiscovered tree species are in South America. Moreover, almost one-third of all tree species to be discovered may be rare, with very low populations and limited spatial distribution (likely in remote tropical lowlands and mountains). These findings highlight the vulnerability of global forest biodiversity to anthropogenic changes in land use and climate, which disproportionately threaten rare species and thus, global tree richness.