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ABSTRACT We present a model for the formation of the Magellanic Stream (MS) due to ram pressure stripping. We model the history of the Small and Large Magellanic Clouds in the recent cosmological past in a static Milky Way (MW) potential with diffuse halo gas, using observationally motivated orbits for the Magellanic Clouds derived from HST proper motions within the potential of the MW. This model is able to reproduce the trailing arm but does not reproduce the leading arm feature, which is common for models of the stream formation that include ram pressure stripping effects. While our model does not outperform other models in terms of matching the observable quantities in the MS, it is close enough for our ultimate goal – using the MS to estimate the MW mass. By analysing our grid of models, we find that there is a direct correlation between the observed stream length in our simulations and the mass of the MW. For the observed MS length, the inferred MW mass is 1.5 ± 0.32 × 1012$$\, \mathrm{M}_\odot$$, which agrees closely with other independent measures of the MW mass. We also discuss the MS in the context of H i streams in galaxy clusters, and find that the MS lies on the low-mass end of a continuum from Hickson groups to the Virgo cluster. As a tracer of the dynamical mass in the outer halo, the MS is a particularly valuable probe of the MW’s potential. 

Abstract Membrane efflux pumps play a major role in bacterial multidrug resistance. The tripartite multidrug efflux pump system fromEscherichia coli, AcrAB-TolC, is a target for inhibition to lessen resistance development and restore antibiotic efficacy, with homologs in other ESKAPE pathogens. Here, we rationalize a mechanism of inhibition against the periplasmic adaptor protein, AcrA, using a combination of hydrogen/deuterium exchange mass spectrometry, cellular efflux assays, and molecular dynamics simulations. We define the structural dynamics of AcrA and find that an inhibitor can inflict long-range stabilisation across all four of its domains, whereas an interacting efflux substrate has minimal effect. Our results support a model where an inhibitor forms a molecular wedge within a cleft between the lipoyl and αβ barrel domains of AcrA, diminishing its conformational transmission of drug-evoked signals from AcrB to TolC. This work provides molecular insights into multidrug adaptor protein function which could be valuable for developing antimicrobial therapeutics. 

The discipline of the humanities has long been inseparable from the exploration of space and time. With the rapid advancement of digitization, databases, and data science, humanities research is making greater use of quantitative spatiotemporal analysis and visualization. In response to this trend, our team developed the Chinese academic map publishing platform (AMAP) with the aim of supporting the digital humanities from a Chinese perspective. In compiling materials mined from China’s historical records, AMAP attempts to reconstruct the geographical distribution of entities including people, activities, and events, using places to connect these historical objects through time. This project marks the beginning of the development of a comprehensive database and visualization system to support humanities scholarship in China, and aims to facilitate the accumulation of spatiotemporal datasets, support multi-faceted queries, and provide integrated visualization tools. The software itself is built on Harvard’s WorldMap codebase, with enhancements which include improved support for Asian projections, support for Chinese encodings, the ability to handle long text attributes, feature level search, and mobile application support. The goal of AMAP is to make Chinese historical data more accessible, while cultivating collaborative opensource software development.