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  1. Abstract
    Foliage was collected in 2015 and 2017 from red maple trees at the Climate Change Across Seasons Experiment (CCASE) as part of the Hubbard Brook Ecosystem Study (HBES). Analyses of foliar metabolites include polyamines, amino acids, chlorophylls, carotenoids, soluble proteins, soluble inorganic elements, sugars, and total nitrogen and carbon. There are six (11 x 14m) plots in total in this study; two control (plots 1 and 2), two warmed 5 degrees (°) Celsius (C) above ambient throughout the growing season (plots 3 and 4), and two warmed 5 °C in the growing season, with snow removal during the winter to induce soil freezing and then warmed with buried heating cables to create a subsequent thaw (plots 5 and 6). Each soil freeze/thaw cycle includes 72 hours of soil freezing followed by 72 hours of thaw. Four kilometers (km) of heating cable are buried in the soil to warm these four plots. Together, these treatments led to warmer growing season soil temperatures and an increased frequency of soil freeze-thaw cycles (FTCs) in winter. Our goal was to determine how these changes in soil temperature affect foliar nitrogen (N) and carbon metabolism of red maple trees. These data were gathered asMore>>