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Creators/Authors contains: "Mertz, Jerome"

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  1. The inherent constraints on resolution, speed and field of view have hindered the development of high-speed, three-dimensional microscopy techniques over large scales. Here, we present a multiplane line-scan imaging strategy, which uses a series of axially distributed reflecting slits to probe different depths within a sample volume. Our technique enables the simultaneous imaging of an optically sectioned image stack with a single camera at frame rates of hundreds of hertz, without the need for axial scanning. We demonstrate the applicability of our system to monitor fast dynamics in biological samples by performing calcium imaging of neuronal activity in mouse brains and voltage imaging of cardiomyocytes in cardiac samples. 
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  3. We present a new type of confocal microscope that simultaneously image a specimen at four different depths, thus providing volumetric imaging at video rate. Our technique is an attractive tool for in vivo high-speed volumetric calcium imaging. 
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  4. We present an alternative illumination scheme for retinal imaging. It is based on near-infrared light delivered transcranially at the temple and light diffusion towards the retina. This unique transmission geometry simplifies absorption measurements and enables clear imaging as deep as the choroid. 
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