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Abstract Porous substrate electroporation (PSEP) is a promising new method for intracellular delivery, yet fundamentals of PSEP are not well understood, especially the intermediate processes leading to delivery. PSEP is an electrical method, yet the relationship between PSEP and electrical impedance remains underexplored. In this study, a device capable of measuring impedance and performing PSEP is developed and the changes in transepithelial electrical impedance (TEEI) are monitored. These measurements show TEEI increases following PSEP, unlike other electroporation methods. The authors then demonstrate how cell culture conditions and electrical waveforms influence this response. More importantly, TEEI response features are correlated with viability and delivery efficiency, allowing prediction of outcomes without fluorescent cargo, imaging, or image processing. This label‐free delivery also allows improved temporal resolution of transient processes following PSEP, which the authors expect will aid PSEP optimization for new cell types and cargos. 

The rheological behaviour of dense suspensions of ideally conductive particles in the presence of both electric field and shear flow is studied using large-scale numerical simulations. Under the action of an electric field, these particles are known to undergo dipolophoresis (DIP), which is the combination of two nonlinear electrokinetic phenomena: induced-charge electrophoresis (ICEP) and dielectrophoresis (DEP). For ideally conductive particles, ICEP is predominant over DEP, resulting in transient pairing dynamics. The shear viscosity and first and second normal stress differences$$N_1$$and$$N_2$$of such suspensions are examined over a range of volume fractions$$15\,\% \leq \phi \leq 50\,\%$$as a function of Mason number$$Mn$$, which measures the relative importance of viscous shear stress over electrokinetic-driven stress. For$$Mn < 1$$or low shear rates, the DIP is shown to dominate the dynamics, resulting in a relatively low-viscosity state. The positive$$N_1$$and negative$$N_2$$are observed at$$\phi < 30\,\%$$, which is similar to Brownian suspensions, while their signs are reversed at$$\phi \ge 30\,\%$$. For$$Mn \ge 1$$, the shear thickening starts to arise at$$\phi \ge 30\,\%$$, and an almost five-fold increase in viscosity occurs at$$\phi = 50\,\%$$. Both$$N_1$$and$$N_2$$are negative for$$Mn \gg 1$$at all volume fractions considered. We illuminate the transition in rheological behaviours from DIP to shear dominance around$$Mn = 1$$in connection to suspension microstructure and dynamics. Lastly, our findings reveal the potential use of nonlinear electrokinetics as a means of active rheology control for such suspensions. 

The transition to turbulence in a plane Poiseuille flow of dilute polymer solutions is studied by direct numerical simulations of a finitely extensible nonlinear elastic fluid with the Peterlin closure. The range of Reynolds number ($$Re$$)$$2000 \le Re \le 5000$$is studied but with the same level of elasticity in viscoelastic flows. The evolution of a finite-amplitude perturbation and its effects on the transition dynamics are investigated. A viscoelastic flow begins transition at an earlier time than its Newtonian counterparts, but the transition time appears to be insensitive to polymer concentration in the dilute and semi-dilute regimes studied. Increasing polymer concentration, however, decreases the maximum attainable energy growth during the transition process. The critical or minimum perturbation amplitude required to trigger transition is computed. Interestingly, both Newtonian and viscoelastic flows follow almost the same power-law scaling of$$Re^\gamma$$with the critical exponent$$\gamma \approx -1.25$$, which is in close agreement with previous studies. However, a shift downward is observed for viscoelastic flow, suggesting that smaller perturbation amplitudes are required for the transition. A mechanism of the early transition is investigated by the evolution of wall-normal and spanwise velocity fluctuations and flow structure. The early growth of these fluctuations and the formation of quasi-streamwise vortices around low-speed streaks are promoted by polymers, hence causing an early transition. These vortical structures are found to support the critical exponent$$\gamma \approx -1.25$$. Once the transition process is completed, polymers play a role in dampening the wall-normal and spanwise velocity fluctuations and vortices to attain a drag-reduced state in viscoelastic turbulent flows. 

Porous substrate electroporation (PSEP) is a promising new method for intracellular delivery, yet fundamentals of PSEP are not well understood, especially the intermediate processes leading to delivery. PSEP is an electrical method, yet the relationship between PSEP and electrical impedance remains underexplored. In this study, a device capable of measuring impedance and performing PSEP is developed and the changes in transepithelial electrical impedance (TEEI) are monitored. These measurements show TEEI increases following PSEP, unlike other electroporation methods. The authors then demonstrate how cell culture conditions and electrical waveforms influence this response. More importantly, TEEI response features are correlated with viability and delivery efficiency, allowing prediction of outcomes without fluorescent cargo, imaging, or image processing. This label-free delivery also allows improved temporal resolution of transient processes following PSEP, which the authors expect will aid PSEP optimization for new cell types and cargos. 

The transition from laminar to turbulent flow is of great interest since it is one of the most difficult and unsolved problems in fluids engineering. The transition processes are significantly important because the transition has a huge impact on almost all systems that come in contact with a fluid flow by altering the mixing, transport, and drag properties of fluids even in simple pipe and channel flows. Generally, in most transportation systems, the transition to turbulence causes a significant increase in drag force, energy consumption, and, therefore, operating cost. Thus, understanding the underlying mechanisms of the laminar-to-turbulent transition can be a major benefit in many ways, especially economically. There have been substantial previous studies that focused on testing the stability of laminar flow and finding the critical amplitudes of disturbances necessary to trigger the transition in various wall-bounded systems, including circular pipes and square ducts. However, there is still no fundamental theory of transition to predict the onset of turbulence. In this study, we perform direct numerical simulations (DNS) of the transition flows from laminar to turbulence in a channel flow. Specifically, the effects of different magnitudes of perturbations on the onset of turbulence are investigated. The perturbation magnitudes vary from 0.001 (0.1%) to 0.05 (5%) of a typical turbulent velocity field, and the Reynolds number is from 5,000 to 40,000. Most importantly, the transition behavior in this study was found to be in good agreement with other reported studies performed for fluid flow in pipes and ducts. With the DNS results, a finite amplitude stability curve was obtained. The critical magnitude of perturbation required to cause transition was observed to be inversely proportional to the Reynolds number for the magnitude from 0.01 to 0.05. We also investigated the temporal behavior of the transition process, and it was found that the transition time or the time required to begin the transition process is inversely correlated with the Reynolds number only for the magnitude from 0.02 to 0.05, while different temporal behavior occurs for smaller perturbation magnitudes. In addition to the transition time, the transition dynamics were investigated by observing the time series of wall shear stress. At the onset of transition, the shear stress experiences an overshoot, then decreases toward sustained turbulence. As expected, the average values of the wall shear stress in turbulent flow increase with the Reynolds number. The change in the wall shear stress from laminar to overshoot was, of course, found to increase with the Reynolds number. More interestingly was the observed change in wall shear stress from the overshoot to turbulence. The change in magnitude appears to be almost insensitive to the Reynolds number and the perturbation magnitude. Because the change in wall shear stress is directly proportional to the pumping power, these observations could be extremely useful when determining the required pumping power in certain flow conditions. Furthermore, the stability curve and wall shear stress changes can be considered robust features for future applications, and ultimately interpreted as evidence of progress toward solving the unresolved fluids engineering problem. 

Non-colloidal suspensions undergoing dipolar interactions in an electric field have been extensively studied and are also known as smart materials as they share similarities with electrorheological (ER) fluids. Although the macroscopic responses are well-documented, the multiscale nature of such suspensions is still lacking. In this study, a large-scale Stokesian dynamics simulation is used to investigate the structural formation of such suspensions in an electric field up to highly concentrated regimes across different length scales: from particle-level (microscale) to particle cluster-level (mesoscale) and stress response-level (macroscale). It is observed that at a volume fraction of ϕ ≈ 30%, the steady-state structures are the most isotropic at the microscale, but at the macroscale, their normal stress fields are the most anisotropic. Interestingly, these structures are also the most heterogeneous at both the microscale and mesoscale. Furthermore, the effects of confinement on the multiscale responses are explored, revealing that there could be a strong link between the mesoscale and macroscale. This multiscale nature can offer the potential for precisely controlling or designing ER fluids in practical applications. 

Direct numerical simulations were performed to study the effects of the domain size of a minimal flow unit (MFU) and its inherent periodic boundary conditions on flow physics of a turbulent channel flow in a range of 200≤Reτ≤1000. This was accomplished by comparing turbulent statistics with those computed in sub-domains (SD) of extended domain simulations. The dimensions of the MFU and SD were matched, and SD dynamics were set to minimize artificial periodicities. Streamwise and spanwise dimensions of healthy MFUs were found to increase linearly with Reynolds number. It was also found that both MFU and SD statistics and dynamics were healthy and in good agreement. This suggests that healthy MFU dynamics represent extended-domain dynamics well up to Reτ=1000, indicating a nearly negligible effect of periodic conditions on MFUs. However, there was a small deviation within the buffer layer for the MFU at Reτ=200, which manifested in an increased mean velocity and a tail in the Q2 quadrant of the u′-v′ plane. Thus, it should be noted that when considering an MFU domain size, stricter criteria may need to be put in place to ensure healthy turbulent dynamics.