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As imaging techniques rapidly evolve to probe nanoscale genome organization at higher resolution, it is critical to consider how the reagents and procedures involved in sample preparation affect chromatin at the relevant length scales. Here, we investigate the effects of fluorescent labeling of DNA sequences within chromatin using the gold standard technique of three-dimensional fluorescencein situhybridization (3D FISH). The chemical reagents involved in the 3D FISH protocol, specifically formamide, cause significant alterations to the sub-200 nm (sub-Mbp) chromatin structure. Alternatively, two labeling methods that do not rely on formamide denaturation, resolution after single-strand exonuclease resection (RASER)-FISH and clustered regularly interspaced short palindromic repeats (CRISPR)-Sirius, had minimal impact on the three-dimensional organization of chromatin. We present a polymer physics-based analysis of these protocols with guidelines for their interpretation when assessing chromatin structure using currently available techniques. 

ABSTRACT Several sources of repeating coherent bursts of radio emission with periods of many minutes have now been reported in the literature. These ‘ultralong period’ (ULP) sources have no clear multiwavelength counterparts and challenge canonical pulsar emission models, leading to debate regarding their nature. In this work, we report the discovery of a bright, highly polarized burst of radio emission at low Galactic latitude as part of a wide-field survey for transient and variable radio sources. ASKAP J175534.9$$-$$252749.1 does not appear to repeat, with only a single intense two-minute $$\sim$$200-mJy burst detected from 60 h of observations. The burst morphology and polarization properties are comparable to those of classical pulsars but the duration is more than one hundred times longer, analogous to ULPs. Combined with the existing ULP population, this suggests that these sources have a strong Galactic latitude dependence and hints at an unexplored population of transient and variable radio sources in the thin disc of the Milky Way. The resemblance of this burst with both ULPs and pulsars calls for a unified coherent emission model for objects with spin periods from milliseconds to tens of minutes. However, whether or not these are all neutron stars or have the same underlying power source remains open for debate. 

ABSTRACT The population of radio-loud stars has to date been studied primarily through either targeted observations of a small number of highly active stars or wide-field, single-epoch surveys that cannot easily distinguish stellar emission from background extragalactic sources. As a result it has been difficult to constrain population statistics such as the surface density and fraction of the population producing radio emission in a particular variable or spectral class. In this paper, we present a sample of 36 radio stars detected in a circular polarization search of the multi-epoch Variables and Slow Transients (VAST) pilot survey with ASKAP at 887.5 MHz. Through repeat sampling of the VAST pilot survey footprint we find an upper limit to the duty cycle of M-dwarf radio bursts of $$8.5 \,\rm {per\,cent}$$, and that at least 10 ± 3 $$\rm {per\,cent}$$ of the population should produce radio bursts more luminous than $$10^{15} \,\rm {erg}\mathrm{s}^{-1} \,\mathrm{Hz}^{-1}$$. We infer a lower limit on the long-term surface density of such bursts in a shallow $$1.25 \,\mathrm{m}\rm {Jy}\rm\ {PSF}^{-1}$$ sensitivity survey of $${9}^{\, +{11}}_{-{7}}\times 10^{-3}$$  $$\,\deg ^{-2}$$ and an instantaneous radio star surface density of 1.7 ± 0.2 × 10−3  $$\,\deg ^{-2}$$ on 12 min time-scales. Based on these rates we anticipate ∼200 ± 50 new radio star detections per year over the full VAST survey and $${41\, 000}^{\, +{10\, 000}}_{-{9\, 000}}$$ in next-generation all-sky surveys with the Square Kilometre Array. 

Abstract We present the Sydney Radio Star Catalogue, a new catalogue of stars detected at megahertz to gigahertz radio frequencies. It consists of 839 unique stars with 3 405 radio detections, more than doubling the previously known number of radio stars. We have included stars from large area searches for radio stars found using circular polarisation searches, cross-matching, variability searches, and proper motion searches as well as presenting hundreds of newly detected stars from our search of Australian SKA Pathfinder observations. The focus of this first version of the catalogue is on objects detected in surveys using SKA precursor and pathfinder instruments; however, we will expand this scope in future versions. The 839 objects in the Sydney Radio Star Catalogue are distributed across the whole sky and range from ultracool dwarfs to Wolf-Rayet stars. We demonstrate that the radio luminosities of cool dwarfs are lower than the radio luminosities of more evolved sub-giant and giant stars. We use X-ray detections of 530 radio stars by the eROSITA soft X-ray instrument onboard the Spectrum Roentgen Gamma spacecraft to show that almost all of the radio stars in the catalogue are over-luminous in the radio, indicating that the majority of stars at these radio frequencies are coherent radio emitters. The Sydney Radio Star Catalogue can be found in Vizier or athttps://radiostars.org. 

Abstract Late-time (∼a year) radio follow-up of optically discovered tidal disruption events (TDEs) is increasingly resulting in detections at radio wavelengths, and there is growing evidence for this late-time radio activity to be common to the broad class of subrelativistic TDEs. Detailed studies of some of these TDEs at radio wavelengths are also challenging the existing models for radio emission. Using all-sky multiepoch data from the Australian Square Kilometre Array Pathfinder (ASKAP), taken as a part of the Rapid ASKAP Continuum Survey (RACS), we searched for radio counterparts to a sample of optically discovered TDEs. We detected late-time emission at RACS frequencies (742–1032 MHz) in five TDEs, reporting the independent discovery of radio emission from TDE AT 2019ahk and extending the time baseline out to almost 3000 days for some events. Overall, we find that at least ${22}_{-11}^{+15}\%$of the population of optically discovered TDEs has detectable radio emission in the RACS survey, while also noting that the true fraction can be higher given the limited cadence (two epochs separated by ∼3 yr) of the survey. Finally, we project that the ongoing higher-cadence (∼2 months) ASKAP Variable and Slow Transients survey can detect ∼20 TDEs in its operational span (4 yr), given the current rate from optical surveys. 

ABSTRACT Large widefield surveys make possible the serendipitous discovery of rare subclasses of pulsars. One such class are ‘spider’-type pulsar binaries, comprised of a pulsar in a compact orbit with a low-mass (sub)stellar companion. In a search for circularly polarized radio sources in Australian Square Kilometre Array Pathfinder (ASKAP) Pilot Survey observations, we discovered highly variable and circularly polarized emission from a radio source within the error region of the γ-ray source 4FGL J1646.5−4406. The variability is consistent with the eclipse of a compact, steep-spectrum source behind ablated material from a companion in an ∼5.3 h binary orbit. Based on the eclipse properties and spatial coincidence with 4FGL J1646.5−4406, we argue that the source is likely a recycled pulsar in a ‘redback’ binary system. Using properties of the eclipses from ASKAP and Murchison Widefield Array observations, we provide broad constraints on the properties of the eclipse medium. We identified a potential optical/infrared counterpart in archival data consistent with a variable low-mass star. Using the Parkes radio telescope ‘Murriyang’ and the Meer Karoo Array Telescope (MeerKAT) , we searched extensively for radio pulsations but yielded no viable detections of pulsed emission. We suggest that the non-detection of pulses is due to scattering in the intra-binary material, but scattering from the interstellar medium can also plausibly explain the pulse non-detections if the interstellar dispersion measure exceeds ∼600 pc cm−3. Orbital constraints derived from optical observations of the counterpart would be highly valuable for future γ-ray pulsation searches, which may confirm the source nature as a pulsar. 

ABSTRACT We present results from a radio survey for variable and transient sources on 15-min time-scales, using the Australian SKA Pathfinder (ASKAP) pilot surveys. The pilot surveys consist of 505 h of observations conducted at around 1 GHz observing frequency, with a total sky coverage of 1476 deg2. Each observation was tracked for approximately 8 – 10 h, with a typical rms sensitivity of ∼30 μJy beam−1 and an angular resolution of ∼12 arcsec. The variability search was conducted within each 8 – 10 h observation on a 15-min time-scale. We detected 38 variable and transient sources. Seven of them are known pulsars, including an eclipsing millisecond pulsar, PSR J2039−5617. Another eight sources are stars, only one of which has been previously identified as a radio star. For the remaining 23 objects, 22 are associated with active galactic nuclei or galaxies (including the five intra-hour variables that have been reported previously), and their variations are caused by discrete, local plasma screens. The remaining source has no multiwavelength counterparts and is therefore yet to be identified. This is the first large-scale radio survey for variables and transient sources on minute time-scales at a sub-mJy sensitivity level. We expect to discover ∼1 highly variable source per day using the same technique on the full ASKAP surveys. 

Abstract We present a systematic search for radio counterparts of novae using the Australian Square Kilometer Array Pathfinder (ASKAP). Our search used the Rapid ASKAP Continuum Survey, which covered the entire sky south of declination $$+41^{\circ}$$ ( $$\sim$$ $34000$ square degrees) at a central frequency of 887.5 MHz, the Variables and Slow Transients Pilot Survey, which covered $$\sim$$ $5000$ square degrees per epoch (887.5 MHz), and other ASKAP pilot surveys, which covered $$\sim$$ 200–2000 square degrees with 2–12 h integration times. We crossmatched radio sources found in these surveys over a two–year period, from 2019 April to 2021 August, with 440 previously identified optical novae, and found radio counterparts for four novae: V5668 Sgr, V1369 Cen, YZ Ret, and RR Tel. Follow-up observations with the Australian Telescope Compact Array confirm the ejecta thinning across all observed bands with spectral analysis indicative of synchrotron emission in V1369 Cen and YZ Ret. Our light-curve fit with the Hubble Flow model yields a value of $$1.65\pm 0.17 \times 10^{-4} \rm \:M_\odot$$ for the mass ejected in V1369 Cen. We also derive a peak surface brightness temperature of $$250\pm80$$ K for YZ Ret. Using Hubble Flow model simulated radio lightcurves for novae, we demonstrate that with a 5 $$\sigma$$ sensitivity limit of 1.5 mJy in 15-min survey observations, we can detect radio emission up to a distance of 4 kpc if ejecta mass is in the range $$10^{-3}\rm \:M_\odot$$ , and upto 1 kpc if ejecta mass is in the range $$10^{-5}$$ – $$10^{-3}\rm \:M_\odot$$ . Our study highlights ASKAP’s ability to contribute to future radio observations for novae within a distance of 1 kpc hosted on white dwarfs with masses $0.4$ – $$1.25\:\rm M_\odot$$ , and within a distance of 4 kpc hosted on white dwarfs with masses $0.4$ – $$1.0\:\rm M_\odot$$ . 

Abstract We present the detection of rotationally modulated, circularly polarized radio emission from the T8 brown dwarf WISE J062309.94−045624.6 between 0.9 and 2.0 GHz. We detected this high-proper-motion ultracool dwarf with the Australian SKA Pathfinder in 1.36 GHz imaging data from the Rapid ASKAP Continuum Survey. We observed WISE J062309.94−045624.6 to have a time and frequency averaged StokesIflux density of 4.17 ± 0.41 mJy beam⁻¹, with an absolute circular polarization fraction of 66.3% ± 9.0%, and calculated a specific radio luminosity ofL_ν∼ 10^14.8erg s⁻¹Hz⁻¹. In follow-up observations with the Australian Telescope Compact Array and MeerKAT we identified a multipeaked pulse structure, used dynamic spectra to place a lower limit ofB> 0.71 kG on the dwarf’s magnetic field, and measured aP= 1.912 ± 0.005 hr periodicity, which we concluded to be due to rotational modulation. The luminosity and period we measured are comparable to those of other ultracool dwarfs observed at radio wavelengths. This implies that future megahertz to gigahertz surveys, with increased cadence and improved sensitivity, are likely to detect similar or later-type dwarfs. Our detection of WISE J062309.94−045624.6 makes this dwarf the coolest and latest-type star observed to produce radio emission. 

Abstract We present the detection of 661 known pulsars observed with the Australian SKA Pathfinder (ASKAP) telescope at 888 MHz as part of the Rapid ASKAP Continuum Survey (RACS). Detections were made through astrometric coincidence and we estimate the false alarm rate of our sample to be ∼0.5%. Using archival data at 400 and 1400 MHz, we estimate the power-law spectral indices for the pulsars in our sample and find that the mean spectral index is −1.78 ± 0.6. However, we also find that a single power law is inadequate for modeling all the observed spectra. With the addition of flux densities between 150 MHz and 3 GHz from various imaging surveys, we find that up to 40% of our sample show deviations from a simple power-law model. Using StokesVmeasurements from the RACS data, we measured the circular polarization fraction for 9% of our sample and find that the mean polarization fraction is ∼10% (consistent between detections and upper limits). Using the dispersion-measure-derived distance, we estimate the pseudo-luminosity of the pulsars and do not find any strong evidence for a correlation with the pulsars’ intrinsic properties.