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The coacervation of alpha‐synuclein (αSyn) into cytotoxic oligomers and amyloid fibrils are considered pathological hallmarks of Parkinson's disease. While aggregation is central to amyloid diseases, liquid–liquid phase separation (LLPS) and its interplay with aggregation have gained increasing interest. Previous work shows that factors promoting or inhibiting aggregation have similar effects on LLPS. This study provides a detailed scanning of a wide range of parameters, including protein, salt and crowding concentrations at multiple pH values, revealing different salt dependencies of aggregation and LLPS. The influence of salt on aggregation under crowding conditions follows a non‐monotonic pattern, showing increased effects at medium salt concentrations. This behavior can be elucidated through a combination of electrostatic screening and salting‐out effects on the intramolecular interactions between the N‐terminal and C‐terminal regions of αSyn. By contrast, this study finds a monotonic salt dependence of LLPS due to intermolecular interactions. Furthermore, it observes time evolution of the two distinct assembly states, with macroscopic fibrillar‐like bundles initially forming at medium salt concentration but subsequently converting into droplets after prolonged incubation. The droplet state is therefore capable of inhibiting aggregation or even dissolving aggregates through heterotypic interactions, thus preventing αSyn from its dynamically arrested state.

 

Abstract. The interaction between storm surge and concurrent precipitation is poorly understood in many coastal regions. This paper investigates the potential compound effects from these two flooding drivers along the coast of China for the first time by using the most comprehensive records of storm surge and precipitation. Statistically significant dependence between flooding drivers exists at the majority of locations that are analysed, but the strength of the correlation varies spatially and temporally and depending on how extreme events are defined. In general, we find higher dependence at the south-eastern tide gauges (TGs) (latitude < 30∘ N) compared to the northern TGs. Seasonal variations in the dependence are also evident. Overall there are more sites with significant dependence in the tropical cyclone (TC) season, especially in the summer. Accounting for past sea level rise further increases the dependence between flooding drivers, and future sea level rise will hence likely lead to an increase in the frequency of compound events. We also find notable differences in the meteorological patterns associated with events where both drivers are extreme versus events where only one driver is extreme. Events with both extreme drivers at south-eastern TG sites are caused by low-pressure systems with similar characteristics across locations, including high precipitable water content (PWC) and strong winds that generate high storm surge. Based on historical disaster damages records of Hong Kong, events with both extreme drivers account for the vast majority of damages and casualties, compared to univariate flooding events, where only one flooding driver occurred. Given the large coastal population and low capacity of drainage systems in many Chinese urban coastal areas, these findings highlight the necessity to incorporate compound flooding and its potential changes in a warming climate into risk assessments, urban planning, and the design of coastal infrastructure and flood defences.